Probe:	Amer2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo	Present

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Diffuse		A gradient of expression along the anterior-posterior axis.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		1C C2
TS19: midbrain	Present		1C C2
TS19: neural tube	Present		1C C2
TS19: telencephalon	Present		1C C2

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		1C C2'

Structure	Level	Pattern	Image	Note
TS15: future brain	Present		2A A1
TS15: neural tube	Present		2A A1
TS15: otocyst	Present		2A A1
TS15: branchial arch	Weak		2A A1

Structure	Level	Pattern	Image	Note
TS15: future brain	Present		2A a

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		2A b

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		2A c
TS15: limb mesenchyme	Moderate	Regionally restricted	2A c	Expression is in the limb bud mesenchyme underlying the surface ectoderm.
TS15: somite	Present	Regionally restricted	2A c	Expression is at the internal edges.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		2B B1
TS17: neural tube	Present		2B B1

Structure	Level	Pattern	Image	Note
TS17: brain	Present		2B a
TS17: dorsal root ganglion	Present		2B a
TS17: neural tube	Present		2B a
TS17: peripheral nerve trunk	Present		2B a
TS17: limb mesenchyme	Moderate	Regionally restricted	2B a	Expression is in the limb bud mesenchyme underlying the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		2B B2
TS17: neural tube	Present		2B B2

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		2B b
TS17: peripheral nerve trunk	Present		2B b
TS17: neural tube lateral wall	Strong	Regionally restricted	2B b	Expression was highest in the marginal layer.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		2B B3
TS17: trigeminal ganglion	Present		2B B3
TS17: branchial arch	Weak		2B B3

Structure	Level	Pattern	Image	Note
TS17: brain	Present		2B c

Structure	Level	Pattern	Image	Note
TS17: hindbrain lateral wall	Present		2B d	Expression is in neuronal groups in the basal plate and alar plate.

Structure	Level	Pattern	Image	Note
TS17: hindbrain lateral wall	Present		2B e	Expression is in neuronal groups in the basal plate and alar plate.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		2B f

Structure	Level	Pattern	Image	Note
TS17: brain	Present		2B inset

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Present		4A Amer2
TS19: dorsal aorta	Present	Regionally restricted	4A Amer2	Expression is in the thoracic dorsal aorta.
TS19: heart	Absent		4A Amer2

Structure	Level	Pattern	Image	Note
TS20: body wall mesenchyme	Present	Regionally restricted	4B Amer2	Expression is in differentiating muscle.
TS20: limb mesenchyme	Present	Regionally restricted	4B Amer2	Expression is in differentiating muscle.
TS20: medulla oblongata	Strong		4B Amer2
TS20: spinal cord	Strong		4B Amer2
TS20: telencephalon	Strong		4B Amer2
TS20: dorsal aorta	Present	Regionally restricted	4B Amer2	Expression is in the thoracic dorsal aorta.
TS20: heart	Absent		4B Amer2

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		4C Amer2
TS21: medulla oblongata	Strong		4C Amer2
TS21: spinal cord	Strong		4C Amer2
TS21: telencephalon	Strong		4C Amer2
TS21: heart	Absent		4C Amer2

Structure	Level	Pattern	Image	Note
TS22: facial ganglion	Present		4D Amer2
TS22: inferior cervical ganglion	Present		4D Amer2
TS22: vestibulo-cochlear ganglion	Present		4D Amer2
TS22: medulla oblongata	Strong		4D Amer2
TS22: midbrain	Strong		4D Amer2
TS22: pons	Strong		4D Amer2
TS22: spinal cord	Strong		4D Amer2
TS22: telencephalon	Strong		4D Amer2
TS22: thalamus	Strong		4D Amer2
TS22: heart	Absent		4D Amer2

Structure	Level	Pattern	Image	Note
TS23: dorsal grey horn	Present		5A Amer2
TS23: dorsal root ganglion	Present		5A Amer2
TS23: ventral grey horn	Present		5A Amer2
TS23: spinal cord marginal layer	Absent		5A Amer2

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		5B Amer2

Structure	Level	Pattern	Image	Note
TS22: inferior cervical ganglion	Present		5C Amer2

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Present		5D Amer2

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present		6A Amer2

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		6B Amer2
TS21: medulla oblongata part of 4th ventricle choroid plexus	Present		6B Amer2

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present		6C Amer2

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present		6D Amer2
TS21: retina	Strong	Spotted	6D Amer2

Structure	Level	Pattern	Image	Note
TS26: retina	Present		6E Amer2

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present		7A Amer2	Expression is in the basal layer.

Structure	Level	Pattern	Image	Note
TS26: olfactory epithelium	Present		7B Amer2	Expression is in the basal layer.
TS26: nose	Strong	Regionally restricted	7B Amer2	Expression is in the tubules of the nasal serous glands.

Structure	Level	Pattern	Image	Note
TS23: vibrissa	Present		7C Amer2

Structure	Level	Pattern	Image	Note
TS23: submandibular gland primordium	Weak		7D Amer2	Weak in the gland but stronger in the presumptive submandibular ganglia along the ducts of the submandibular gland.

Structure	Level	Pattern	Image	Note
TS20: lung	Weak		7E Amer2

Structure	Level	Pattern	Image	Note
TS23: tongue	Absent		7F Amer2

Structure	Level	Pattern	Image	Note
TS22: comma-shaped body	Weak		8A Amer2
TS22: s-shaped body	Weak		8A Amer2
TS22: adrenal medulla	Present	Spotted	8A Amer2

Structure	Level	Pattern	Image	Note
TS22: comma-shaped body	Weak		8B Amer2
TS22: s-shaped body	Weak		8B Amer2

Structure	Level	Pattern	Image	Note
TS22: testis	Weak	Single cells	8C Amer2	Expression is in a subset of cells of the interstitial population.

Structure	Level	Pattern	Image	Note
TS22: ovary	Weak	Regionally restricted	8D Amer2	Expression is in the cortical region.

Structure	Level	Pattern	Image	Note
TS22: bladder	Present	Regionally restricted	8E Amer2	Faint expression was observed in the future bladder wall. However, strong expression was detected in the bladder neck.

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Present	Regionally restricted	9i	Expression is in neurons of the suprarenal ganglion.
TS22: adrenal medulla	Present	Spotted	9i

Structure	Level	Pattern	Image	Note
TS20: peripheral nerve trunk	Present		9j
TS20: dorsal aorta	Present	Regionally restricted	9j	Expression is in the thoracic dorsal aorta.

Structure	Level	Pattern	Image	Note
TS22: hypogastric plexus	Present		9k

Structure	Level	Pattern	Image	Note
TS14: future brain	Strong		S1B Amer2
TS14: embryo	Strong	Regionally restricted	S1B Amer2	Expression is in anterior regions.

Structure	Level	Pattern	Image	Note
TS14: future brain	Strong		S1B inset

Structure	Level	Pattern	Image	Note
TS19: metanephric mesenchyme	Present	Regionally restricted		Expresssion is in the metanephric mesenchyme surrounding the branching epithelium.

Structure	Level	Pattern	Image	Note
TS20: intraembryonic coelom	Weak	Regionally restricted		Expression is in the coelomic epithelium of the male gonad.
TS20: forelimb pre-cartilage condensation	Weak	Regionally restricted		Expression is in the epiphyseal region of the long bones, whereas the ossifying diaphyseal region was virtually devoid of staining.

Structure	Level	Pattern	Image	Note
TS21: thymus primordium	Absent

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Absent