Probe:	Wtx/Amer1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo	Present

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Diffuse		A gradient of expression along the anterior-posterior axis.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		1A
TS15: future brain	Present		1A
TS15: neural tube	Present		1A
TS15: optic cup	Present		1A
TS15: otocyst	Present		1A
TS15: tail unsegmented mesenchyme	Present		1A
TS15: head mesenchyme	Present	Regionally restricted	1A	Expression is in the craniofacial mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: branchial arch mesenchyme	Present		1Aa
TS15: future brain	Present		1Aa
TS15: otocyst	Present		1Aa

Structure	Level	Pattern	Image	Note
TS15: intraembryonic coelom	Very strong		1Ab
TS15: umbilical artery	Very strong		1Ab
TS15: umbilical vein	Very strong		1Ab
TS15: trunk mesenchyme	Very strong	Regionally restricted	1Ab	Expression is in the body wall.
TS15: future brain	Present		1Ab
TS15: neural tube	Present		1Ab
TS15: somite	Present	Regionally restricted	1Ab	Expression is at the internal edges.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		1Ac
TS15: somite	Present	Regionally restricted	1Ac	Expression is at the internal edges.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	1A inset	Expression is at the internal edges.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		1Ba
TS17: neural tube	Present		1Ba
TS17: limb mesenchyme	Strong	Regionally restricted	1Ba	Expression is in the limb bud mesenchyme underlying the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS17: intraembryonic coelom	Very strong		1Bb
TS17: umbilical artery	Very strong		1Bb
TS17: umbilical vein	Very strong		1Bb
TS17: trunk mesenchyme	Very strong	Regionally restricted	1Bb	Expression is in the body wall.
TS17: brain	Present		1Bb
TS17: branchial arch mesenchyme	Present		1Bb
TS17: neural tube	Present		1Bb
TS17: trigeminal ganglion	Present		1Bb

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		1C C1
TS19: midbrain	Present		1C C1
TS19: neural tube	Present		1C C1
TS19: telencephalon	Present		1C C1

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		1C C1'

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Strong		4A Wtx/Amer1
TS19: intraembryonic coelom	Strong	Regionally restricted	4A Wtx/Amer1	Expression is in the coelomic epithelium of the urogenital ridge.
TS19: heart	Absent		4A Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS20: body wall mesenchyme	Present	Regionally restricted	4B Wtx/Amer1	Expression is in differentiating muscle.
TS20: limb mesenchyme	Present	Regionally restricted	4B Wtx/Amer1	Expression is in differentiating muscle.
TS20: spinal cord	Present		4B Wtx/Amer1
TS20: telencephalon	Present		4B Wtx/Amer1
TS20: medulla oblongata	Present		4B Wtx/Amer1	Strong in the neuronal tracts.
TS20: heart	Absent		4B Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		4C Wtx/Amer1
TS21: medulla oblongata	Present		4C Wtx/Amer1
TS21: spinal cord	Present		4C Wtx/Amer1
TS21: telencephalon	Present		4C Wtx/Amer1
TS21: heart	Absent		4C Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS22: facial ganglion	Present		4D Wtx1/Amer1
TS22: inferior cervical ganglion	Present		4D Wtx1/Amer1
TS22: midbrain	Present		4D Wtx1/Amer1
TS22: pons	Present		4D Wtx1/Amer1
TS22: spinal cord	Present		4D Wtx1/Amer1
TS22: telencephalon	Present		4D Wtx1/Amer1
TS22: thalamus	Present		4D Wtx1/Amer1
TS22: vestibulo-cochlear ganglion	Present		4D Wtx1/Amer1
TS22: medulla oblongata	Present		4D Wtx1/Amer1	Strong in the neuronal tracts.
TS22: heart	Absent		4D Wtx1/Amer1

Structure	Level	Pattern	Image	Note
TS23: dorsal grey horn	Present		5A WTX/Amer1
TS23: dorsal root ganglion	Present		5A WTX/Amer1
TS23: ventral grey horn	Present		5A WTX/Amer1
TS23: spinal cord marginal layer	Absent		5A WTX/Amer1

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		5B WTX/Amer1

Structure	Level	Pattern	Image	Note
TS22: inferior cervical ganglion	Present		5C WTX/Amer1

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Present		5D WTX/Amer1

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present		6A Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		6B Wtx/Amer1
TS21: medulla oblongata part of 4th ventricle choroid plexus	Present		6B Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present		6C Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present		6D Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present		6E Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present		7A Wtx/Amer1	Expression is in the presumptive supporting cell layer.

Structure	Level	Pattern	Image	Note
TS26: olfactory epithelium	Present		7B Wtx/Amer1	Expression is in the presumptive supporting cell layer.
TS26: nose	Present	Regionally restricted	7B Wtx/Amer1	Expression is in the tubules of the nasal serous glands.

Structure	Level	Pattern	Image	Note
TS23: vibrissa	Present		7C Wtx/Amer1	Expression is in the epithelial compartment, predominantly in the connective tissue sheath.
TS23: epidermis	Present		7C Wtx/Amer1	Expression is in the interfollicular epidermis.

Structure	Level	Pattern	Image	Note
TS23: submandibular gland primordium	Present		7D Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present		7E Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS23: primary palate epithelium	Present		7F Wtx/Amer1
TS23: tongue intrinsic muscle	Present		7F Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS22: comma-shaped body	Present		8A Wtx/Amer1
TS22: s-shaped body	Present		8A Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS22: renal cortex	Present		8B Wtx/Amer1
TS22: comma-shaped body	Present		8B Wtx/Amer1
TS22: s-shaped body	Present		8B Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS22: testis	Present	Regionally restricted	8C Wtx/Amer1	Expression is in interstitial tissue.

Structure	Level	Pattern	Image	Note
TS22: surface epithelium of ovary	Strong		8D Wtx/Amer1

Structure	Level	Pattern	Image	Note
TS22: bladder	Present	Regionally restricted	8E Wtx/Amer1	Expression is in the muscularis mucosa and external adventicia layer, whereas much lower expression was seen in the smooth muscle layers.

Structure	Level	Pattern	Image	Note
TS22: forelimb interdigital region	Very strong	Regionally restricted	9a	Expression is right below the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present		9a left section

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present		9a middle section

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present		9a right section

Structure	Level	Pattern	Image	Note
TS22: forelimb musculature	Strong		9b
TS22: forelimb cartilage condensation	Moderate	Regionally restricted	9b	Expression is in the epiphyseal region of the long bones, whereas the ossifying diaphyseal region was virtually devoid of staining.

Structure	Level	Pattern	Image	Note
TS22: femur cartilage condensation	Present	Regionally restricted	9b1	Expression is in the epiphyseal region of the long bones, whereas the ossifying diaphyseal region was virtually devoid of staining.

Structure	Level	Pattern	Image	Note
TS22: femur cartilage condensation	Present	Regionally restricted	9b2	Expression is in the epiphyseal region of the long bones, whereas the ossifying diaphyseal region was virtually devoid of staining.

Structure	Level	Pattern	Image	Note
TS22: primary palate epithelium	Present		9c
TS22: tongue intrinsic muscle	Present		9c

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present		9d	Strong in the neuronal tracts.

Structure	Level	Pattern	Image	Note
TS23: lung	Present		9e

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present		9e inset	Expression is in the epithelium of the terminal bronchi.

Structure	Level	Pattern	Image	Note
TS22: esophagus epithelium	Present		9f
TS22: lung epithelium	Present		9f
TS22: main bronchus epithelium	Present		9f

Structure	Level	Pattern	Image	Note
TS20: umbilical artery extraembryonic part	Present		9g
TS20: umbilical vein extraembryonic part	Present		9g

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Trace	Regionally restricted	9h	A signal above background levels was observed at the level of the cortical region.

Structure	Level	Pattern	Image	Note
TS14: embryo	Present		S1B WTX

Structure	Level	Pattern	Image	Note
TS17: genitourinary system	Present			Expression is in the urogenital ridge.

Structure	Level	Pattern	Image	Note
TS19: metanephric mesenchyme	Present	Regionally restricted		Expresssion is in the metanephric mesenchyme surrounding the branching epithelium.

Structure	Level	Pattern	Image	Note
TS20: gonad	Present			both male and female
TS20: mesonephros	Absent
TS20: nephric duct	Absent
TS20: paramesonephric duct	Absent

Structure	Level	Pattern	Image	Note
TS21: thymus primordium	Absent