Ccnd1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type:	RNA in situ
MGI Accession ID:	MGI:4450850
Gene symbol:	Ccnd1
Gene name:	cyclin D1

Probe:	UI-M-BH3-asm-e-05-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

S9_v ©

Specimen S9v: embryonic day 11.5 (more

)
Note: Double labeled: blue - Ccnd1; brown - Shh (assay MGI:4452154).

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S9_v	Expression is present in mitotic progenitors.

S9_w ©

Specimen S9w: embryonic day 12.5 (more

)
Note: Double labeled: blue - Ccnd1; brown - Shh (assay MGI:4452154).

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	S9_w	Expression is present in mitotic progenitors.

S9_x ©

Specimen S9x: embryonic day 14.5 (more

)
Note: Double labeled: blue - Ccnd1; brown - Shh (assay MGI:4452154).

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	S9_x	Expression is present in mitotic progenitors.

S9_y ©

Specimen S9y: embryonic day 16.5 (more

)
Note: Double labeled: blue - Ccnd1; brown - Shh (assay MGI:4452154).

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	S9_y	Expression is present in mitotic progenitors.

CYCLIND1(D)+SHH(F)-E11.5-CORONAL ©

Specimen Ccnd1 - E11.5: embryonic day 11.5 (more

)
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Ambiguous		CYCLIND1(D)+SHH(F)-E11.5-CORONAL	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer	Very strong	Non-Uniform	CYCLIND1(D)+SHH(F)-E11.5-CORONAL	Staining is very strong in the VZ of the dorsal & ventral POAs & anterior & posterior IDs, strong in dorsal thalamus, PVH, VMH & ARH nuclei, premam zone & TT, moderate in the AVH & mam & supramam zones & moderate & scattered in the prethalamus.
TS19: telencephalon mantle layer	Weak	Non-Uniform	CYCLIND1(D)+SHH(F)-E11.5-CORONAL	There is weak, scattered staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex.
TS19: telencephalon ventricular layer	Strong	Non-Uniform	CYCLIND1(D)+SHH(F)-E11.5-CORONAL	There is strong staining in the ventricular zone of the ganglionic eminences and moderate staining in the cerebral cortex.

Specimen Ccnd1 - E12.5: embryonic day 12.5 (more

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		CYCLIND1(D)+SHH(F)-E12.5-CORONAL	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Moderate		CYCLIND1(D)+SHH(F)-E12.5-CORONAL	There is moderate staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		CYCLIND1(D)+SHH(F)-E12.5-CORONAL	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Ambiguous		CYCLIND1(D)+SHH(F)-E12.5-CORONAL	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer	Very strong	Non-Uniform	CYCLIND1(D)+SHH(F)-E12.5-CORONAL	There is very strong staining in the ventricular zone of the premam, mam and supramam zones and strong staining in the TT.
TS20: ganglionic eminence	Strong	Non-Uniform	CYCLIND1(D)+SHH(F)-E12.5-CORONAL	There is strong staining in the ventricular zone of the ganglionic eminences and weak, scattered staining in the mantle zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Ambiguous		CYCLIND1(D)+SHH(F)-E12.5-CORONAL	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Very strong	Non-Uniform	CYCLIND1(D)+SHH(F)-E12.5-CORONAL	There is very strong staining in the ventricular zone of the dorsal and ventral POAs, strong staining in the AVH and VMH and ARH nuclei and moderate staining in the anterior and posterior IDs and moderate, scattered staining in the PVH nucleus.
TS20: thalamus mantle layer	Ambiguous		CYCLIND1(D)+SHH(F)-E12.5-CORONAL	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Very strong		CYCLIND1(D)+SHH(F)-E12.5-CORONAL	There is very strong staining in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Ccnd1 - E14.5: embryonic day 14.5 (more

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		CYCLIND1(D)+SHH(F)-E14.5-CORONAL	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Moderate		CYCLIND1(D)+SHH(F)-E14.5-CORONAL	There is moderate staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		CYCLIND1(D)+SHH(F)-E14.5-CORONAL	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		CYCLIND1(D)+SHH(F)-E14.5-CORONAL	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Very strong	Non-Uniform	CYCLIND1(D)+SHH(F)-E14.5-CORONAL	There is very strong staining in the ventricular zone of the mam zone and weak, scattered staining in the premam and supramam zones and the TT.
TS22: ganglionic eminence	Moderate	Non-Uniform	CYCLIND1(D)+SHH(F)-E14.5-CORONAL	There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS22: hypothalamus mantle layer	Ambiguous		CYCLIND1(D)+SHH(F)-E14.5-CORONAL	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Very strong	Non-Uniform	CYCLIND1(D)+SHH(F)-E14.5-CORONAL	Staining is very strong in the VZ of the ARH nucleus, strong in the ventral POA, the AVH & the VMH nucleus, weak in the PVH nucleus & anterior ID & weak & scattered in the dorsal POA. Staining is either absent or ubiquitous in the VZ of posterior ID.
TS22: thalamus mantle layer	Ambiguous		CYCLIND1(D)+SHH(F)-E14.5-CORONAL	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Strong	Non-Uniform	CYCLIND1(D)+SHH(F)-E14.5-CORONAL	There is strong staining in the ventricular zone of the dorsal thalamus and weak staining in the prethalamus.

Specimen Ccnd1 - E16.5: embryonic day 16.5 (more

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		CYCLIND1(D)+SHH(F)-E16.5-CORONAL	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		CYCLIND1(D)+SHH(F)-E16.5-CORONAL	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		CYCLIND1(D)+SHH(F)-E16.5-CORONAL	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		CYCLIND1(D)+SHH(F)-E16.5-CORONAL	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer	Strong	Non-Uniform	CYCLIND1(D)+SHH(F)-E16.5-CORONAL	There is strong staining in the ventricular zone of the mam zone. Staining is either absent or ubiquitous in the ventricular zone of the premam and supramam zones.
TS24: ganglionic eminence	Weak	Non-Uniform	CYCLIND1(D)+SHH(F)-E16.5-CORONAL	There is weak staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS24: hypothalamus mantle layer	Ambiguous		CYCLIND1(D)+SHH(F)-E16.5-CORONAL	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer	Strong	Non-Uniform	CYCLIND1(D)+SHH(F)-E16.5-CORONAL	There is strong staining in the ventricular zone of the ARH nucleus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Ambiguous		CYCLIND1(D)+SHH(F)-E16.5-CORONAL	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Ambiguous		CYCLIND1(D)+SHH(F)-E16.5-CORONAL	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

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