Probe:	Lin-28 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Ubiquitous	1a
TS15: eye	Strong		1a
TS15: forelimb bud	Strong		1a
TS15: hindlimb ridge	Strong		1a
TS15: otocyst	Strong		1a

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong		1a'
TS15: future hindbrain	Strong		1a'
TS15: future midbrain	Strong		1a'

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Strong		1b
TS15: neural tube	Strong		1b
TS15: forelimb bud mesenchyme	Present	Regionally restricted	1b	Expression is present in the distal mesenchyme, underneath the apical ectodermal ridge.
TS15: hindlimb ridge mesenchyme	Present	Regionally restricted	1b	Expression is present in the distal mesenchyme, underneath the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS15: hindlimb ridge mesenchyme	Present	Regionally restricted	1b'	Expression is present in the distal mesenchyme, underneath the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present		1c
TS17: forelimb bud	Absent		1c	Expression has either disappeared or almost disappeared.
TS17: trunk	Present	Regionally restricted	1c	Expression is present in the more caudal trunk, at the hindlimb level.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		1d
TS17: hindlimb bud	Present		1d
TS17: trunk	Present	Regionally restricted	1d	Expression is present in the more caudal trunk, at the hindlimb level.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present		1d'	Expression is present in the most distal mesenchyme, underneath the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present		1e
TS17: neural tube	Present		1e
TS17: trunk	Present	Regionally restricted	1e	Expression is present in the more caudal trunk, at the hindlimb level.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present		1f
TS17: eye	Present		1f
TS17: midbrain	Present		1f

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		1g
TS19: hindlimb bud	Trace		1g	Expression is barely detectable.
TS19: eye	Present		1g

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		1h	Expression is present at the apical edge.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		2E E9.5 FL

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Absent		2E E10.5 FL

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		2E E11.5 FL

Structure	Level	Pattern	Image	Note
TS15: hindlimb ridge	Present		2E E9.5 HL

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present		2E E10.5 HL

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Absent		2E E11.5 HL