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Gene Expression Data
Assay Details
Assay
Reference: J:31131 Pfaff SL, et al., Requirement for LIM homeobox gene Isl1 in motor neuron generation reveals a motor neuron-dependent step in interneuron differentiation. Cell. 1996 Jan 26;84(2):309-20
Assay type: Immunohistochemistry
MGI Accession ID: MGI:4360053
Gene symbol: En1
Gene name: engrailed 1
Antibody: anti-EN1
Results
Specimen 6A: embryonic day 9.5 (more )
Note: This is caudal section. Double labeled: green - En1; red - Isl1 (assay MGI:4360055 and Isl2 (assay MGI:4360104).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Absent 6A At this level in the embryo, there is no expression in the ventral spinal cord.
Specimen 6B: embryonic day 9.5 (more )
Note: This is a rostral section. Double labeled: green - En1; red - Isl1 (assay MGI:4360055 and Isl2 (assay MGI:4360104).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Present Regionally restricted 6B At this level in the embryo, expression is present in interneurons close to the motor neurons in the ventral spinal cord.
Specimen 6C: embryonic day 10.5 (more )
Note: This is a rostral section. Double labeled: green - En1; red - Isl1 (assay MGI:4360055 and Isl2 (assay MGI:4360104).
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 6C Expression is present in interneurons located close to a dorsal cluster of motor neurons.
Specimen 6D: embryonic day 15.5 (more )
Note: This is a rostral section. Double labeled: green - En1; red - Isl1 (assay MGI:4360055 and Isl2 (assay MGI:4360104).
Structure Level Pattern Image Note
TS23: spinal cord Present Regionally restricted 6D Expression is present in the interneurons in the ventral spinal cord; they have intermingled with the motor neurons.
Specimen 6E: embryonic day 9.5; Isl1tm1Tmj/Isl1tm1Tmj (more )
Note: This is a 25-somite stage embryo. This is a rostral section. Double labeled: green - En1; red - Isl1 (assay MGI:4360055 and Isl2 (assay MGI:4360104).
Structure Level Pattern Image Note
TS15: future spinal cord Absent 6E
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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