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Gene Expression Data
Assay Details
Assay
Reference: J:31131 Pfaff SL, et al., Requirement for LIM homeobox gene Isl1 in motor neuron generation reveals a motor neuron-dependent step in interneuron differentiation. Cell. 1996 Jan 26;84(2):309-20
Assay type: Immunohistochemistry
MGI Accession ID: MGI:4360055
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: anti-Isl1/2
Assay notes: The antibody used in this assay recognizes two related proteins.
Results
Specimen 6A: embryonic day 9.5 (more )
Note: This is caudal section. Double labeled: red - Isl1 and Isl2; green - En1 (assay MGI:4360053).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Present Regionally restricted 6A Expression is present in the motor neurons of the ventral spinal cord.
Specimen 6B: embryonic day 9.5 (more )
Note: This is a rostral section. Double labeled: red - Isl1 and Isl2; green - En1 (assay MGI:4360053).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Present Regionally restricted 6B Expression is present in the motor neurons of the ventral spinal cord.
Specimen 6C: embryonic day 10.5 (more )
Note: This is a rostral section. Double labeled: red - Isl1 and Isl2; green - En1 (assay MGI:4360053).
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 6C Expression is present in motor neurons.
Specimen 6D: embryonic day 15.5 (more )
Note: This is a rostral section. Double labeled: red - Isl1 and Isl2; green - En1 (assay MGI:4360053).
Structure Level Pattern Image Note
TS23: spinal cord Present Regionally restricted 6D Expression is present in the motor neurons.
Specimen 6E: embryonic day 9.5; Isl1tm1Tmj/Isl1tm1Tmj (more )
Note: This is a 25-somite stage embryo. Double labeled: red - Isl1 and Isl2; green - En1 (assay MGI:4360053).
Structure Level Pattern Image Note
TS15: future spinal cord Absent 6E
Specimen 6I: embryonic day 9.5 (more )
Note: Double labeled: red - Isl1 and Isl2; green - mpm-2 (for mitotic cells).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Present 6I Expression is not present in mitotic cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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