Cpa3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:18760 Yamazaki M, et al., C-kit gene is expressed by skin mast cells in embryos but not in puppies of Wsh/Wsh mice: age-dependent abolishment of c-kit gene expression. Blood. 1994 Jun 15;83(12):3509-16
Assay type:	RT-PCR
MGI Accession ID:	MGI:4360048
Gene symbol:	Cpa3
Gene name:	carboxypeptidase A3, mast cell

Probe:	MC-CPA-pA, MC-CPA-pB
Assay notes:	The RT-PCR reaction was blotted and hybridized to a cDNA probe.

Results

Image: 2_MC-CPA PC18

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	689 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
Lane 1	E17.5 (a)	TS25: skin	Present	5 µg; total RNA	C57BL/6		Not Specified
Lane 2	E17.5 (a)	TS25: skin	Present (b)	0.5 µg; total RNA	C57BL/6		Not Specified
Lane 3	E17.5 (a)	TS25: skin	Not Specified	0.05 µg; total RNA	C57BL/6		Not Specified
Lane 4	E17.5 (a)	TS25: skin	Not Specified	0.005 µg; total RNA	C57BL/6		Not Specified
Lane 5	E17.5 (a)	TS25: skin	Present (b)	5 µg; total RNA	involves: C57BL/6	Kit^W-sh/Kit^W-sh	Not Specified
Lane 6	E17.5 (a)	TS25: skin	Not Specified	0.5 µg; total RNA	involves: C57BL/6	Kit^W-sh/Kit^W-sh	Not Specified
Lane 7	E17.5 (a)	TS25: skin	Not Specified	0.05 µg; total RNA	involves: C57BL/6	Kit^W-sh/Kit^W-sh	Not Specified
Lane 8	E17.5 (a)	TS25: skin	Not Specified	0.005 µg; total RNA	involves: C57BL/6	Kit^W-sh/Kit^W-sh	Not Specified

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) The magnitude of signal at 5ug RNA from mutant skin was comparable with that of the signal from 0.5ug RNA from +/+ skin.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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