Antibody:	Anti-Grin1
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS28: accessory olfactory bulb	Present		7S , 7G	Expression is present in almost all layers. It might be localized to axons and dendrites.
TS28: main olfactory bulb	Present		7S , 7G	Expression is present in almost all layers. It might be localized to axons and dendrites.
TS28: accessory olfactory bulb glomerular layer	Weak		7G , 7S
TS28: main olfactory bulb glomerular layer	Weak		7G , 7S
TS28: main olfactory bulb mitral cell layer	Weak		7G , 7S

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Present	Non-Uniform	7T , 7H	Expression is present in regions surrounding the pyramidal cell somata, suggesting expression in axons and dendrites.

Structure	Level	Pattern	Image	Note
TS28: amygdala	Weak		7U , 7I
TS28: basolateral amygdaloid nucleus	Present		7U , 7I	This is one of the areas of strongest expression.
TS28: lateral amygdaloid nucleus	Present		7U , 7I	This is one of the areas of strongest expression.
TS28: medial amygdaloid nucleus	Present		7U , 7I	This is one of the areas of strongest expression.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		7V , 7J	Expression is present in most layers.
TS28: dentate gyrus	Strong		7V , 7J	Expression is present in most layers.
TS28: hippocampus CA1	Strong		7V , 7J
TS28: hippocampus CA2	Present		7V , 7J
TS28: hippocampus CA3	Present		7V , 7J
TS28: hippocampus stratum oriens	Present	Non-Uniform	7V , 7J	In CA1, expression is strong in these layers; in CA2/3 it is weaker.
TS28: hippocampus stratum radiatum	Present	Non-Uniform	7V , 7J	In CA1, expression is strong in these layers; in CA2/3 it is weaker.
TS28: dentate gyrus molecular layer	Strong		7V , 7J
TS28: hippocampus molecular layer	Weak		7J , 7V
TS28: hippocampus stratum lacunosum	Weak		7J , 7V

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		7K , 7W	Expression is present without any layer or region specificity.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		7X , 7L	This is the where the predominant expression is.
TS28: cerebellum molecular layer	Moderate		7X , 7L

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present		8A , 8G	Expression is present in neurons but primarily outside the neuronal cell somata.

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Present		8H , 8B	Expression is present in neurons but primarily outside the neuronal cell somata. Faint staining was observed in the cell somata.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		8C , 8I	Expression is present in neurons but primarily outside the neuronal cell somata.