Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:138310
Xu C, et al., Expression of Robo/Slit and Semaphorin/Plexin/Neuropilin family members in the developing hypothalamic paraventricular and supraoptic nuclei. Gene Expr Patterns. 2008 Sep;8(7-8):502-7
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:3845300
|
Gene symbol:
|
Slit2
|
Gene name:
|
slit guidance ligand 2
|
Probe:
|
Slit2 cDNA3
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
Assay notes:
|
Fixative was Methancarn (60% methanol,
30% chloroform, and 10% acetic acid).
|
|
Results
|
Specimen
2E1, E5:
embryonic day 12.5
(more )
Note: (E5) is a higher magnification of the boxed area in (E1).
Specimen
2E1, E5:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
(E5) is a higher magnification of the boxed area in (E1).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon ventricular layer |
Present |
Regionally restricted |
2_E5
,
2_E1
|
Expression is in a punctate pattern next to the paraventricular nucleus/supraoptic nucleus progenitor.
|
TS20: hypothalamus |
Present |
Regionally restricted |
2_E5
,
2_E1
|
Expression is in a small group of cells at the dorsal-medial portion of the paraventricular nucleus/supraoptic nucleus progenitor.
|
Specimen
2E2:
embryonic day 13.5
(more )
Specimen
2E2:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Specimen
2E3:
embryonic day 14.5
(more )
Specimen
2E3:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Specimen
2E4:
embryonic day 15.5
(more )
Specimen
2E4:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
|