Probe:	mNFI-B cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS14: embryo	Absent

Structure	Level	Pattern	Image	Note
TS17: lung	Present

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	3B	Expression is present in the neocortex.
TS19: brain ventricular layer	Present		3B
TS19: 1st branchial arch	Present		3B	Expression is present throughout the mandibular arch.
TS19: lung	Present		3B
TS19: liver	Present		3B
TS19: gut mesenchyme	Present		3B

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted		Expression is present in the ependymal layer of the neural tube.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Non-Uniform		Expression was the same as was described for E11.5.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Non-Uniform	4B	Expression was the same as was described for E11.5.
TS22: embryo mesenchyme	Present	Regionally restricted	4B	Expresson is predominantly around developing cartilage and in muscle connective tissues.
TS22: nasal cavity epithelium	Weak		4B	There are low levels of expression in the sinus epithelium.
TS22: limb interdigital region	Present		4B
TS22: lung	Present		4B
TS22: neocortex	Very strong		4B
TS22: cerebellum	Strong	Regionally restricted	4B	Strong expression is present in migrating neurons.
TS22: spinal cord	Strong	Regionally restricted	4B	Strong expression is present in migrating neurons.
TS22: cranial ganglion	Present	Regionally restricted	4B	Expression is present in a punctate fashion, suggesting it is localized in glial cells.
TS22: dorsal root ganglion	Present	Regionally restricted	4B	Expression is present in a punctate fashion, suggesting it is localized in glial cells.

Structure	Level	Pattern	Image	Note
TS24: pons	Strong		5B	There is high expression in pontine nuclei.
TS24: cerebral cortex	Strong		5B
TS24: skin	Present	Regionally restricted	5B	Expression is present around the whisker follicles.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	6B	Expression is predominantly in the white matter, suggesting it is expressed by glia.
TS28: dentate gyrus	Present		6B	Expression levels in the dentate gyrus are higher than in the horn of Ammon cells.
TS28: hippocampus	Present		6B

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present
TS28: cerebral cortex	Present