Gene Expression Data
Assay Details
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Assay
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Reference:
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J:64329
Welsh IC, et al., Loss of late primitive streak mesoderm and interruption of left-right morphogenesis in the Ednrb(s-1Acrg) mutant mouse. Dev Biol. 2000 Sep 1;225(1):151-68
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3837224
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Tissues were fixed in 0.2% glutaraldehyde.
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Results
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Specimen
8K WT:
embryonic day 8.0; Nodaltm1Rob/Nodal +
(more  )
Note: 4-7 somite stage.
Specimen
8K WT:
(close  )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Note:
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4-7 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: notochordal plate |
Present |
Regionally restricted |
8K WT
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Expression was detected along the periphery of the node and funneled evenly into the notochordal plate.
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| TS12: primitive streak |
Present |
Regionally restricted |
8K WT
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Expression was detected along the periphery of the node and funneled evenly into the notochordal plate.
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| TS12: notochord |
Present |
Regionally restricted |
8K WT
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Occasional cells were detected extending into the notochord.
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Specimen
8K -/-:
(close )
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Genetic Background:
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involves: 101/Rl * 129S/SvEv * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Glutaraldehyde
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Note:
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4-7 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: notochordal plate |
Weak |
Regionally restricted |
8K -/-
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Compared to wild type, marked cells were reduced in number and unevenly distributed along the lateral edges of the notochordal plate.
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| TS12: notochord |
Present |
Regionally restricted |
8K -/-
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Compared to wild type, there were significantly fewer labeled cells in the notochord.
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| TS12: primitive streak |
Present |
Regionally restricted |
8K -/-
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Labeled cells accumulated at the posterior end of the node.
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Analysis Tools
