Vgll3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:144198 Mielcarek M, et al., VITO-2, a new SID domain protein, is expressed in the myogenic lineage during early mouse embryonic development. Gene Expr Patterns. 2009 Mar;9(3):129-37
Assay type:	RNA in situ
MGI Accession ID:	MGI:3833404
Gene symbol:	Vgll3
Gene name:	vestigial like family member 3

Probe:	pSPORT6-VITO-2
Probe preparation:	Antisense RNA

Results

3A ©

Specimen 3A: embryonic day 8.5 (more

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Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		3A

3B ©

Specimen 3B: embryonic day 8.5 (more

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Note: Vibratome section.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		3B

3C ©

Specimen 3C: embryonic day 9.5 (more

)

Structure	Level	Image	Note
TS15: somite	Present	3C	The first somite that showed clear expression was somite VI.
TS15: somite 06	Present	3C	The first somite that showed clear expression was somite VI.
TS15: branchial arch	Absent	3C
TS15: future midbrain	Absent	3C
TS15: limb	Absent	3C

3D ©

Specimen 3D: embryonic day 9.5 (more

)
Note: Vibratome section.

Structure	Level	Pattern	Image	Note
TS15: trunk myotome	Present		3D
TS15: trunk dermomyotome	Present	Regionally restricted	3D	Absent in the dermatome.
TS15: limb	Absent		3D
TS15: notochord	Absent		3D
TS15: trunk sclerotome	Absent		3D

3E ©

Specimen 3E: embryonic day 10.5 (more

)

Structure	Level	Image	Note
TS17: somite	Present	3E	The first somite with expression was somite VI.
TS17: somite 06	Present	3E	The first somite with expression was somite VI.
TS17: branchial arch	Absent	3E
TS17: limb	Absent	3E
TS17: midbrain	Absent	3E

3F ©

Specimen 3F: embryonic day 10.5 (more

)
Note: Vibratome section.

Structure	Level	Pattern	Image	Note
TS17: trunk myotome	Present		3F
TS17: limb	Absent		3F

3G ©

Specimen 3G: embryonic day 11.5 (more

)

Structure	Level	Image
TS19: somite	Present	3G
TS19: branchial arch	Absent	3G
TS19: limb	Absent	3G
TS19: midbrain	Absent	3G

3H ©

Specimen 3H: embryonic day 11.5 (more

)
Note: Vibratome section.

Structure	Level	Pattern	Image	Note
TS19: trunk myotome	Present		3H
TS19: limb	Absent		3H

4A ©

4B ©

4C ©

4D ©

4E ©

Specimen 4A-E: embryonic day 12.5 (more

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Note: (B-E) are higher magnifications of areas in (A).

Structure	Level	Pattern	Image	Note
TS20: nervous system	Present		4A , 4B , 4E , 4C , 4D	Expression is throughout the neuroepithelium.
TS20: head	Present	Regionally restricted	4C , 4D , 4E , 4B , 4A	Expression is in the mandibular arch.
TS20: mandibular process	Present	Regionally restricted	4C , 4D , 4E , 4B , 4A	Expression is in the mandibular arch.
TS20: dorsal root ganglion	Present		4A , 4C , 4B , 4E , 4D
TS20: hindbrain	Present		4A , 4C , 4B , 4E , 4D
TS20: heart	Absent		4B , 4E , 4C , 4D , 4A

4F ©

4G ©

4H ©

4I ©

4J ©

Specimen 4F-J: embryonic day 13.5 (more

)
Note: (G-J) are higher magnifications of areas in (F).

Structure	Level	Pattern	Image	Note
TS21: nervous system	Present		4G , 4J , 4H , 4I , 4F	Expression is throughout the neuroepithelium.
TS21: dorsal root ganglion	Present		4I , 4H , 4G , 4J , 4F
TS21: hindbrain	Present		4I , 4H , 4G , 4J , 4F
TS21: head	Present	Regionally restricted	4I , 4J , 4G , 4H , 4F	Expression is in the mandibular arch.
TS21: heart	Absent		4I , 4G , 4H , 4J , 4F

5A ©

Specimen 5A: embryonic day 15.5 (more

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Structure	Level	Pattern	Image	Note
TS23: shoulder	Present	Regionally restricted	5A	Expression is in the shoulder muscle.
TS23: skeletal muscle	Present	Regionally restricted	5A	Expression is in the shoulder muscle.
TS23: anterior abdominal wall muscle	Present		5A

5B ©

Specimen 5B: embryonic day 15.5 (more

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Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted	5B	Expression is in the dorsal part of the neural tube.

5C ©

Specimen 5C: embryonic day 15.5 (more

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Structure	Level	Pattern	Image	Note
TS23: brain	Present		5C

5D ©

Specimen 5D: embryonic day 15.5 (more

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Structure	Level	Pattern	Image	Note
TS23: skeletal muscle	Present		5D

5E ©

Specimen 5E: embryonic day 15.5 (more

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Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted	5E	Expression is in the dorsal part of the neural tube.

5F ©

Specimen 5F: embryonic day 15.5 (more

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Structure	Level	Pattern	Image	Note
TS23: brainstem	Present		5F

5G ©

Specimen 5G: embryonic day 17.5 (more

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Structure	Level	Image
TS25: peripheral nerve trunk	Strong	5G
TS25: spinal cord	Strong	5G
TS25: skeletal muscle	Weak	5G

5H ©

Specimen 5H: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: brainstem	Strong		5H
TS25: inner ear vestibular component	Strong		5H

6A ©

6B ©

Specimen 6A,B: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: heart atrium	Present	Regionally restricted	6B , 6A	Expression is in the atrial area containing different nerve ganglia.
TS28: cardiac muscle tissue	Absent		6B , 6A

7_I A ©

Specimen 7 IA: postnatal adult (more

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Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		7_I A

7_I B ©

Specimen 7 IB: postnatal adult (more

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Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		7_I B
TS28: cerebellum granule cell layer	Strong		7_I B

7_I C ©

Specimen 7 IC: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		7_I C
TS28: cerebral cortex	Present		7_I C
TS28: forebrain	Present	Regionally restricted	7_I C	Expression is in subcortical nuclei.

7_I D ©

Specimen 7 ID: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		7_I D
TS28: forebrain	Present	Regionally restricted	7_I D	Expression is in subcortical nuclei.

7_I E ©

Specimen 7 IE: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		7_I E

7_I F ©

Specimen 7 IF: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		7_I F

8C ©

Specimen 8C: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: trunk myotome	Present		8C

8D ©

Specimen 8D: embryonic day 10.5; Myf5^tm1Jae/Myf5^tm1Jae (more

)

Structure	Level	Pattern	Image	Note
TS17: trunk myotome	Absent		8D

9C ©

Specimen 9C: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: tail	Absent		9C

9D ©

Specimen 9D: embryonic day 10.5; Dll1^tm1Gos/Dll1^tm1Gos (more

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Structure	Level	Pattern	Image	Note
TS17: tail	Present		9D

9E ©

Specimen 9E: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: tail	Absent		9E

9F ©

Specimen 9F: embryonic day 10.5; Dll1^tm1Gos/Dll1^tm1Gos (more

)

Structure	Level	Pattern	Image	Note
TS17: tail	Present		9F

9G ©

Specimen 9G: embryonic day 10.5 (more

)
Note: Vibratome section of (E).

Structure	Level	Pattern	Image	Note
TS17: neural tube	Absent		9G

9H ©

Specimen 9H: embryonic day 10.5; Dll1^tm1Gos/Dll1^tm1Gos (more

)
Note: Vibratome section of (F).

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	9H	Expression is in the caudal neural tube.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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