Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:133341
Vives J, et al., A mouse model for tracking nigrostriatal dopamine neuron axon growth. Genesis. 2008 Mar;46(3):125-31
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:3793603
|
|
Gene symbol:
|
Pitx3
|
|
Gene name:
|
paired-like homeodomain transcription factor 3
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
|
Results
|
Specimen
1b:
embryonic day 14.5; Pitx3tm2Mli/Pitx3 +
(more  )
Specimen
1b:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1c:
embryonic day 16.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
1c:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: midbrain |
Present |
Regionally restricted |
1c
|
Expression in midbrain dopaminergic neurons.
|
Specimen
2a:
embryonic day 11.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
2a:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2b:
embryonic day 12.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
2b:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
2b
|
Expression in two cell clusters along the midline of the ventral mesencephalon.
|
Specimen
2c:
embryonic day 13.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
2c:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: brain |
Present |
Regionally restricted |
2c
|
Increase in both expression intensity and size of expression domain, with expression in apparent neuronal processes projecting rostrally.
|
Specimen
2d:
embryonic day 14.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
2d:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
Regionally restricted |
2d
|
Segregation of expression to cells that correspond to the ventral tegmental area and substantia nigra dopaminergic neurons in the mesencephalic flexure.
|
Specimen
2e:
embryonic day 15.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
2e:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: brain |
Present |
Regionally restricted |
2e
|
Expression in ventral tegmental area cells positioned medial-caudally along the longitudinal axis and substantia nigra dopaminergic neurons extending lateral-rostrally in three cell clusters.
|
Specimen
2f:
embryonic day 16.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
2f:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Present |
Regionally restricted |
2f
|
Authors report that a further expansion of the expression domain was observed.
|
Specimen
2g:
embryonic day 18.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
2g:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3a:
embryonic day 13.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
3a:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
Regionally restricted |
3a
|
Expression in neuron projections traversing ventral-laterally and rostrally along the immature medial forebrain bundle ventral to the thalamus.
|
Specimen
3b:
embryonic day 14.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
3b:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
3b
|
Expression in neuron projections traversing ventral-laterally and rostrally along the immature medial forebrain bundle ventral to the thalamus.
|
Specimen
3c:
embryonic day 16.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
3c:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: midbrain |
Present |
Regionally restricted |
3c
|
Expression in projections which have reached the posterior border of the striatum.
|
Specimen
3d:
embryonic day 18.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
3d:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Present |
Regionally restricted |
3d
|
Expression in projections; authors state the athe anterior border of expression neuron fibers is at an equivalent anatomical position in the striatum as that seen at E16.5.
|
Specimen
3e:
embryonic day 13.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
3e:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: brain |
Present |
Regionally restricted |
3e
|
Expression in neuron projections traversing ventral-laterally and rostrally along the immature medial forebrain bundle ventral to the thalamus.
|
Specimen
3f:
embryonic day 14.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
3f:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
Regionally restricted |
3f
|
Expression in neuron projections traversing ventral-laterally and rostrally along the immature medial forebrain bundle ventral to the thalamus.
|
Specimen
3g:
embryonic day 16.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
3g:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Present |
Regionally restricted |
3g
|
Expression in projections which have reached the posterior border of the striatum.
|
Specimen
3h:
embryonic day 18.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
3h:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain |
Present |
Regionally restricted |
3h
|
Expression in projections; authors state the athe anterior border of expression neuron fibers is at an equivalent anatomical position in the striatum as that seen at E16.5.
|
Specimen
5a:
embryonic day 14.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
5a:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
Regionally restricted |
5a
|
Expression in midbrain dopaminergic axon tracts.
|
Specimen
5b:
embryonic day 16.5; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
5b:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Present |
Regionally restricted |
5b
|
|
Specimen
5c:
postnatal day 3; Pitx3tm2Mli/Pitx3 +
(more )
Specimen
5c:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3+
|
|
Age:
|
postnatal day 3
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: brain |
Present |
Regionally restricted |
5c
|
Expression in midbrain dopaminergic axon tracts.
|
Specimen
5d:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3tm2Mli
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
Regionally restricted |
5d
|
Authors state there was no aberrant expression in midbrain dopaminergic axon tracts.
|
Specimen
5e:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3tm2Mli
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Present |
Regionally restricted |
5e
|
Authors state there was no aberrant expression in midbrain dopaminergic axon tracts.
|
Specimen
5f:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Pitx3tm2Mli/Pitx3tm2Mli
|
|
Age:
|
postnatal day 3
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: brain |
Present |
Regionally restricted |
5f
|
Authors state there was no aberrant expression in midbrain dopaminergic axon tracts.
|
|
Analysis Tools
