Probe:	clone 31
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo	Present

Structure	Level	Pattern	Image	Note
TS13: intermediate mesenchyme	Present	Regionally restricted	3A	Expression in a region adjacent to 10 of the 11 somites present.

Structure	Level	Pattern	Image	Note
TS13: genitourinary system	Present	Regionally restricted	3B	Strongest staining between the level of the somites 8-11 in the area of the presumptive mesonephric region.
TS13: nephrogenic cord	Present		3B	Expression in the nephrogenic cord.

Structure	Level	Pattern	Image	Note
TS15: nephric duct	Present		3C	Expression in the mesonephric duct.

Structure	Level	Pattern	Image	Note
TS15: nephric duct	Present		3D	Expression in the mesonephric duct.

Structure	Level	Pattern	Image	Note
TS15: mesonephros	Present		3E	Globular expression ventral to the mesonephric duct, corresponding to the developing mesonephros.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Absent

Structure	Level	Pattern	Image	Note
TS17: nephric duct, mesonephric portion	Weak		3F	Expression in mesonephric duct is less intense than at E9.5.
TS17: mesonephros	Present		3F	Localized expression ventral to the duct in the developing mesonephros.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	3G	Expression in distinct dorsal and lateral regions.
TS17: hindbrain	Present	Regionally restricted	3G	In the dorsolateral region, a single stripe of expression anterior to the otic vesicle, with two stripes posterior to the otic vesicle.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	3H	Expression in distinct dorsal and lateral regions.
TS17: hindbrain	Present	Regionally restricted	3H	Continuous weak expression spanning the dorsolateral regions of expression, connecting the stripes identified in Fig. 3G. In the ventromedial region, fine bands of perpendicular expression are connected by a longitudinal stripe of faint expression.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	3I

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	3J (left)	Four strips of expressing cells, arranged in 2 tightly apposed pairs dorsally and laterally.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	3J (right)	Four strips of expressing cells, arranged in 2 tightly apposed pairs dorsally and laterally.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Weak

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present		3K	Expression in the telencephalon becomes more prominent

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Strong		3L

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Present		5A
TS19: metanephros	Present	Regionally restricted	5A	No expression in the proximal portion of the ureter or in the mesenchymal tissue around the bud.

Structure	Level	Pattern	Image	Note
TS21: ureter	Present	Regionally restricted	5B	Localized expression in areas coinciding with the position of the buds of the branching ureter.

Structure	Level	Pattern	Image	Note
TS21: ureter	Present	Regionally restricted	5C	Expression in distal regions of the branching ureter.
TS21: primitive collecting duct	Absent		5C

Structure	Level	Pattern	Image	Note
TS23: ureter	Present	Regionally restricted	5D	Expression in distal regions of the branching ureter.
TS23: collecting duct	Absent		5D

Structure	Level	Pattern	Image	Note
TS23: ovary	Absent
TS23: female reproductive system	Absent			No expression in the oviduct.

Structure	Level	Pattern	Image	Note
TS23: testis	Absent
TS23: male reproductive system	Absent			No expression in the epididymis.

Structure	Level	Pattern	Image	Note
TS24: ureter	Present	Regionally restricted	5E	Expression in distal regions of the branching ureter.