Tfap2a RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:128903 Feng W, et al., Identification and analysis of a conserved Tcfap2a intronic enhancer element required for expression in facial and limb bud mesenchyme. Mol Cell Biol. 2008 Jan;28(1):315-25
Assay type:	RNA in situ
MGI Accession ID:	MGI:3789144
Gene symbol:	Tfap2a
Gene name:	transcription factor AP-2, alpha

Probe:	TRIP1
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

Specimen 5A WT: embryonic day 10.5 (more

)

Structure	Level	Image
TS17: brain	Present	5A WT
TS17: branchial arch	Present	5A WT
TS17: dorsal root ganglion	Present	5A WT
TS17: eye	Present	5A WT
TS17: forelimb bud	Present	5A WT
TS17: future spinal cord	Present	5A WT
TS17: hindlimb bud	Present	5A WT

Specimen 5A DCE -/-: embryonic day 10.5; Tfap2a^tm3Will/Tfap2a^tm3Will (more

)

Structure	Level	Image	Note
TS17: brain	Present	5A DCE -/-	Expression similar to that seen in wild-type embryos.
TS17: branchial arch	Present	5A DCE -/-	Expression similar to that seen in wild-type embryos.
TS17: dorsal root ganglion	Present	5A DCE -/-	Expression similar to that seen in wild-type embryos.
TS17: eye	Present	5A DCE -/-	Expression similar to that seen in wild-type embryos.
TS17: future spinal cord	Present	5A DCE -/-	Expression similar to that seen in wild-type embryos.
TS17: forelimb bud	Weak	5A DCE -/-	Expression reduced compared with wild-type embryos.
TS17: hindlimb bud	Weak	5A DCE -/-	Expression reduced compared with wild-type embryos.

Specimen 5B WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	5B WT

Specimen 5B DCE -/-: embryonic day 10.5; Tfap2a^tm3Will/Tfap2a^tm3Will (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Weak	Regionally restricted	5B DCE -/-	Reduced expression compared with wild-type. Expression only in a posterior domain that was more limited than in the wild-type. Anterior and medial expression domains were essentially abolished.

Specimen 5C WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present	Regionally restricted	5C WT

Specimen 5C DCE -/-: embryonic day 10.5; Tfap2a^tm3Will/Tfap2a^tm3Will (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Weak	Regionally restricted	5C DCE -/-	Reduced expression compared with wild-type. Expression only in a posterior domain that was more limited than in the wild-type. Anterior and medial expression domains were essentially abolished.

Specimen 5D WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: latero-nasal process mesenchyme	Present		5D WT
TS17: medial-nasal process mesenchyme	Present		5D WT

Specimen 5D DCE -/-: embryonic day 10.5; Tfap2a^tm3Will/Tfap2a^tm3Will (more

)

Structure	Level	Pattern	Image	Note
TS17: latero-nasal process mesenchyme	Weak		5D DCE -/-	Lower expression compared with wild-type.
TS17: medial-nasal process mesenchyme	Weak		5D DCE -/-	Lower expression compared with wild-type.

Specimen 6A WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud ectoderm	Present		6A WT
TS17: forelimb bud mesenchyme	Present		6A WT

Specimen 6A DCE -/-: embryonic day 10.5; Tfap2a^tm3Will/Tfap2a^tm3Will (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud ectoderm	Present		6A DCE -/-	Expression in ectoderm similar to that seen in wild-type.
TS17: forelimb bud mesenchyme	Trace		6A DCE -/-	Expression in mesenchyme almost completely abolished.

Specimen 6B WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud ectoderm	Present		6B WT
TS17: hindlimb bud mesenchyme	Present		6B WT

Specimen 6B DCE -/-: embryonic day 10.5; Tfap2a^tm3Will/Tfap2a^tm3Will (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud ectoderm	Present		6B DCE -/-	Expression in ectoderm similar to that seen in wild-type.
TS17: hindlimb bud mesenchyme	Trace		6B DCE -/-	Expression in mesenchyme almost completely abolished.

Specimen 6C WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: head	Present	Regionally restricted	6C WT	Expression in surface ectoderm of the head and in the facial mesenchyme.
TS17: head mesenchyme	Present	Regionally restricted	6C WT	Expression in surface ectoderm of the head and in the facial mesenchyme.
TS17: surface ectoderm	Present	Regionally restricted	6C WT	Expression in surface ectoderm of the head and in the facial mesenchyme.
TS17: trigeminal ganglion	Present		6C WT

Specimen 6C DCE -/-: embryonic day 10.5; Tfap2a^tm3Will/Tfap2a^tm3Will (more

)

Structure	Level	Pattern	Image	Note
TS17: head	Present	Regionally restricted	6C DCE -/-	Expression similar to that seen in wild-type.
TS17: surface ectoderm	Present	Regionally restricted	6C DCE -/-	Expression similar to that seen in wild-type.
TS17: trigeminal ganglion	Present		6C DCE -/-	Expression similar to that seen in wild-type.
TS17: head mesenchyme	Weak	Regionally restricted	6C DCE -/-	Lower expression in facial mesenchyme compared with wild-type.

Specimen 6D WT: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: head	Present	Regionally restricted	6D WT	Expression in the surface ectoderm of the head and in the facial mesenchyme.
TS17: head mesenchyme	Present	Regionally restricted	6D WT	Expression in the surface ectoderm of the head and in the facial mesenchyme.
TS17: surface ectoderm	Present	Regionally restricted	6D WT	Expression in the surface ectoderm of the head and in the facial mesenchyme.

Specimen 6D DCE -/-: embryonic day 10.5; Tfap2a^tm3Will/Tfap2a^tm3Will (more

)

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Weak	Regionally restricted	6D DCE -/-	Lower expression in facial mesenchyme compared with wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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