Probe:	Parp9 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	No significant difference in the expression patterns was observed between male and female embryos.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		1B
TS17: gut	Present		1B
TS17: cloaca	Strong		1B
TS17: telencephalon	Strong		1B

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Strong	Uniform	1E
TS22: cerebral cortex	Present		1E
TS22: duodenum	Present		1E
TS22: epithelium of urogenital sinus	Present		1E
TS22: hypothalamus	Present		1E
TS22: intervertebral disc	Present		1E
TS22: main olfactory bulb	Present		1E
TS22: mesenchyme of urogenital sinus	Present		1E
TS22: midgut loop	Present		1E
TS22: nasal cavity	Present		1E
TS22: oral epithelium	Present		1E
TS22: pituitary gland	Present		1E
TS22: primary palate epithelium	Present		1E
TS22: rectum	Present		1E
TS22: telencephalon	Present		1E
TS22: intestine	Present	Regionally restricted	1E	Expression is in the posterior part of the gut (duodenum, jejunum, ileum, colon, rectum).

Structure	Level	Pattern	Image	Note
TS25: thymus	Strong	Uniform	1H
TS25: cerebral cortex	Present		1H inset , 1H
TS25: main olfactory bulb	Present		1H inset , 1H
TS25: nasal cavity	Present		1H inset , 1H
TS25: telencephalon	Present		1H inset , 1H
TS25: hypothalamus	Weak	Regionally restricted	1H	Expression is ventral.
TS25: adenohypophysis pars anterior	Weak		1H

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Strong	Uniform	2B

Structure	Level	Pattern	Image	Note
TS26: thymus	Present	Graded	2E	Highest expression is in the cortex.
TS26: thymus medullary core	Present	Graded	2E	Highest expression is in the cortex.

Structure	Level	Pattern	Image	Note
TS28: thymus medullary core	Strong		2H

Structure	Level	Pattern	Image	Note
TS28: spleen white pulp	Moderate		2K
TS28: spleen red pulp	Moderate	Regionally restricted	2K	Expression is in the marginal zone.

Structure	Level	Pattern	Image	Note
TS28: Peyer's patch	Moderate		2N
TS28: lymph node follicle	Moderate		2N
TS28: large intestine mucosa	Strong	Regionally restricted	2N	Expression is in the follicle associated epithelium.
TS28: small intestine epithelium	Strong	Regionally restricted	2N	Expression is in the follicle associated epithelium.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		3B
TS20: hypothalamus	Present		3B
TS20: nasal cavity epithelium	Present		3B
TS20: telencephalon	Present		3B

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		3E
TS28: hippocampus pyramidal cell layer	Present		3E
TS28: cerebral cortex	Absent		3E

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3H

Structure	Level	Pattern	Image	Note
TS20: intestine	Present		4B
TS20: midgut loop	Present		4B
TS20: urogenital sinus	Present		4B

Structure	Level	Pattern	Image	Note
TS26: colon	Present		4E	Expression is throughout the entire villi.
TS26: ileum	Present		4E	Expression is throughout the entire villi.

Structure	Level	Pattern	Image	Note
TS28: ileum	Present	Regionally restricted	4G	Expression is in enterocytes. Expression is slightly higher from the bottom of the crypt to the crypt-villus junction, and gradually decreases toward more differentiated cells along the axis of the villi.

Structure	Level	Pattern	Image	Note
TS28: colon	Present	Regionally restricted	4I	Expression is in enterocytes.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Weak	Regionally restricted		Expression is ventral.
TS20: esophagus	Absent
TS20: stomach	Absent

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Weak	Regionally restricted		Expression is ventral.
TS22: adenohypophysis pars anterior	Weak
TS22: esophagus	Absent
TS22: stomach	Absent

Structure	Level	Pattern	Image	Note
TS25: gut	Present	Regionally restricted		Expression is in the posterior part of the gut (duodenum, jejunum, ileum, colon, rectum).

Structure	Level	Pattern	Image	Note
TS26: gut	Present	Regionally restricted		Expression is in the posterior part of the gut (duodenum, jejunum, ileum, colon, rectum).
TS26: hypothalamus	Present	Regionally restricted		Expression is ventral.
TS26: adenohypophysis pars anterior	Weak
TS26: esophagus	Absent
TS26: stomach	Absent

Structure	Level	Pattern	Image	Note
TS28: duodenum	Present	Regionally restricted		Expression is in enterocytes.
TS28: jejunum	Present	Regionally restricted		Expression is in enterocytes.