Probe:	MH1492 probe 1198
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Weak		3B Sucla2
TS22: midbrain	Weak		3B Sucla2
TS22: cerebellum	Weak		3B Sucla2
TS22: pons	Weak		3B Sucla2
TS22: medulla oblongata	Weak		3B Sucla2
TS22: spinal cord	Moderate		3B Sucla2
TS22: cranial ganglion	Moderate		3B Sucla2
TS22: dorsal root ganglion	Moderate		3B Sucla2
TS22: olfactory epithelium	Moderate		3B Sucla2
TS22: intestine	Weak		3B Sucla2
TS22: salivary gland	Moderate		3B Sucla2
TS22: tongue	Moderate		3B Sucla2
TS22: stomach	Weak		3B Sucla2
TS22: liver	Weak		3B Sucla2
TS22: lung	Moderate		3B Sucla2
TS22: metanephros	Moderate		3B Sucla2
TS22: thyroid gland	Weak		3B Sucla2
TS22: pituitary gland	Weak		3B Sucla2
TS22: pancreas	Weak		3B Sucla2
TS22: adrenal gland	Moderate		3B Sucla2
TS22: heart	Weak		3B Sucla2
TS22: skeletal muscle	Moderate		3B Sucla2
TS22: cartilage	Absent		3B Sucla2	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3B Sucla2	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Present		MH1492_3A , MH1492_4A
TS22: central nervous system	Present		MH1492_3A , MH1492_4A
TS22: liver	Present		MH1492_3A , MH1492_4A
TS22: metanephros	Present		MH1492_3A , MH1492_4A
TS22: peripheral nervous system	Present		MH1492_3A , MH1492_4A
TS22: salivary gland	Present		MH1492_3A , MH1492_4A
TS22: skeletal muscle	Present		MH1492_3A , MH1492_4A
TS22: alimentary system	Present		MH1492_3A , MH1492_4A	Expression is present in the gastrointestinal tract.
TS22: cartilage	Absent		MH1492_4A , MH1492_3A	There is no expression in the axial cartilage.
TS22: limb cartilage condensation	Absent		MH1492_3A , MH1492_4A