Probe:	MH1052 probe 913
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		3A Pkm2
TS22: midbrain	Moderate		3A Pkm2
TS22: cerebellum	Moderate		3A Pkm2
TS22: pons	Moderate		3A Pkm2
TS22: medulla oblongata	Moderate		3A Pkm2
TS22: spinal cord	Strong		3A Pkm2
TS22: cranial ganglion	Moderate		3A Pkm2
TS22: dorsal root ganglion	Moderate		3A Pkm2
TS22: olfactory epithelium	Strong		3A Pkm2
TS22: intestine	Strong		3A Pkm2
TS22: salivary gland	Strong		3A Pkm2
TS22: tongue	Moderate		3A Pkm2
TS22: stomach	Strong		3A Pkm2
TS22: liver	Moderate		3A Pkm2
TS22: lung	Strong		3A Pkm2
TS22: metanephros	Strong		3A Pkm2
TS22: thyroid gland	Strong		3A Pkm2
TS22: pituitary gland	Strong		3A Pkm2
TS22: pancreas	Strong		3A Pkm2
TS22: adrenal gland	Moderate		3A Pkm2
TS22: heart	Strong		3A Pkm2
TS22: skeletal muscle	Strong		3A Pkm2
TS22: cartilage	Absent		3A Pkm2	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Pkm2	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Present	Non-Uniform	MH1052_4A , MH1052_2D	Expression is ubiquitous in the central nervous system but is elevated in certain structures.
TS22: brain ventricular layer	Present	Non-Uniform	MH1052_4A , MH1052_2D	Expression is elevated in the ventricular zone that lines the left ventricles.
TS22: inferior colliculus	Present		MH1052_2D , MH1052_4A	Expression is elevated in this structure.
TS22: embryo	Present	Regionally restricted	MH1052_4A , MH1052_2D	Many tissues outside the central nervous system express this gene.
TS22: vertebral pre-cartilage condensation	Absent		MH1052_4A , MH1052_2D
TS22: liver	Ambiguous		MH1052_4A , MH1052_2D	In the liver, most cells do not express, with the exception of blood vessels.
TS22: blood vessel	Present		MH1052_2D , MH1052_4A	Expression is present in blood vessels of the liver.