Probe:	MH2013 probe 1678
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		3A Gapdh
TS22: midbrain	Strong		3A Gapdh
TS22: cerebellum	Strong		3A Gapdh
TS22: pons	Strong		3A Gapdh
TS22: medulla oblongata	Strong		3A Gapdh
TS22: spinal cord	Strong		3A Gapdh
TS22: cranial ganglion	Strong		3A Gapdh
TS22: dorsal root ganglion	Strong		3A Gapdh
TS22: olfactory epithelium	Strong		3A Gapdh
TS22: intestine	Strong		3A Gapdh
TS22: salivary gland	Strong		3A Gapdh
TS22: tongue	Strong		3A Gapdh
TS22: stomach	Strong		3A Gapdh
TS22: liver	Strong		3A Gapdh
TS22: lung	Strong		3A Gapdh
TS22: metanephros	Strong		3A Gapdh
TS22: thyroid gland	Strong		3A Gapdh
TS22: pituitary gland	Strong		3A Gapdh
TS22: pancreas	Strong		3A Gapdh
TS22: adrenal gland	Strong		3A Gapdh
TS22: heart	Strong		3A Gapdh
TS22: skeletal muscle	Moderate		3A Gapdh
TS22: cartilage	Absent		3A Gapdh	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Gapdh	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Regionally restricted	MH2013_4C	Expression is present throughout the embryo, except in the axial skeletal elements.
TS22: axial skeleton	Absent		MH2013_4C