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Gene Expression Data
Assay Details
Assay
Reference: J:95540 Chen A, et al., Mouse corticotropin-releasing factor receptor type 2alpha gene: isolation, distribution, pharmacological characterization and regulation by stress and glucocorticoids. Mol Endocrinol. 2005 Feb;19(2):441-58
Assay type: RT-PCR
MGI Accession ID: MGI:3765982
Gene symbol: Crhr2
Gene name: corticotropin releasing hormone receptor 2
Probe: Crhr2-pJ, Crhr2-pK
Assay notes: Semiquantitative RT-PCR assay followed by Southern hybridization analysis. The relative density of specific bands, quantified and normalized relative to the S16 expression are presented in Figure 3C.
Results Image: 3B mCRFR2alpha
 Sample Information Bands Other Sample Information
Lane AgeStructure 418 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
DNA Marker Mol. Wt. Marker Lane
Pancreas P adult TS28: pancreas Absent 0.2 µg; total RNA C57BL/6 Not Specified
Pituitary P adult TS28: pituitary gland Absent 0.2 µg; total RNA C57BL/6 Not Specified
MamG-Vrg P adult TS28: mammary gland Absent 0.2 µg; total RNA C57BL/6 Female Mammary gland of virgin.
MamG-Lac10 P adult TS28: mammary gland Absent 0.2 µg; total RNA C57BL/6 Female Mammary gland of day 10 lactation.
MamG-Lac21 P adult TS28: mammary gland Absent 0.2 µg; total RNA C57BL/6 Female Mammary gland of day 21 lactation.
2D Brain P2 TS27: brain Present 0.2 µg; total RNA C57BL/6 Not Specified
Placenta E12 E12.0 (a) TS20: extraembryonic component
TS28: placenta 		Absent 0.2 µg; total RNA C57BL/6 Not Specified
Placenta E18 E18.0 (a) TS26: extraembryonic component
TS28: placenta 		Absent 0.2 µg; total RNA C57BL/6 Not Specified
Embryo E12 E12.0 (a) TS20: embryo Absent 0.2 µg; total RNA C57BL/6 Not Specified
Total Brain P adult TS28: brain Present 0.2 µg; total RNA C57BL/6 Not Specified
Olfactory bulb P adult TS28: main olfactory bulb Present 0.2 µg; total RNA C57BL/6 Not Specified
Hypothalamus P adult TS28: hypothalamus Present 0.2 µg; total RNA C57BL/6 Not Specified
Cortex P adult TS28: cerebral cortex Present 0.2 µg; total RNA C57BL/6 Not Specified
Cerebellum P adult TS28: cerebellum Present 0.2 µg; total RNA C57BL/6 Not Specified
Hippocampus P adult TS28: hippocampus Present 0.2 µg; total RNA C57BL/6 Not Specified
Midbrain P adult TS28: midbrain Present 0.2 µg; total RNA C57BL/6 Not Specified
Pons/Medulla P adult TS28: medulla oblongata
TS28: pons 		Present 0.2 µg; total RNA C57BL/6 Not Specified
Spinal cord P adult TS28: spinal cord Present 0.2 µg; total RNA C57BL/6 Not Specified
-cDNA Control
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory