Gene Expression Data
Assay Details
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Assay
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Reference:
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J:85300
Helmbacher F, et al., Met signaling is required for recruitment of motor neurons to PEA3-positive motor pools. Neuron. 2003 Aug 28;39(5):767-77
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3763238
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Gene symbol:
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Etv4
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Gene name:
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ets variant 4
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Results
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Specimen
2D:
embryonic day 11.5
(more  )
Note: 49 somites. Double labeled: red - Etv4; green - Isl1 (assay MGI:3763237) and Isl2 (assay MGI:3763239).
Specimen
2D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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49 somites. Double labeled: red - Etv4; green - Isl1 (assay MGI:3763237) and Isl2 (assay MGI:3763239).
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Specimen
2E:
(close )
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Genetic Background:
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C57BL/6J-Pax3Sp-d
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Mutant Allele(s):
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Pax3Sp-d/Pax3Sp-d
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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49 somites. Double labeled: red - Etv4; green - Isl1 (assay MGI:3763237) and Isl2 (assay MGI:3763239).
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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49 somites. Double labeled: red - Etv4; green - Isl1 (assay MGI:3763237) and Isl2 (assay MGI:3763239).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
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2F
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The pool of immunolabeled motor neurons was reduced by 40% in the mutant spinal cord.
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Specimen
4:
embryonic day 11.5
(more )
Note: 49 somites. The data in Fig. 4 was from a set of serial sections along the anteroposterior axis of the brachial spinal cord. This data was presented graphically.
Specimen
4:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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49 somites. The data in Fig. 4 was from a set of serial sections along the anteroposterior axis of the brachial spinal cord. This data was presented graphically.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
4
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Expression was detected in the C5-C8 region of the spinal cord.
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Specimen
4:
embryonic day 11.5; Mettm1Cpo/ Mettm1Cpo
(more )
Note: 49 somites. The data in Fig. 4 was from a set of serial sections along the anteroposterior axis of the brachial spinal cord. This data was presented graphically.
Specimen
4:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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49 somites. The data in Fig. 4 was from a set of serial sections along the anteroposterior axis of the brachial spinal cord. This data was presented graphically.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
4
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The number of neurons was significantly reduced in the mutant. The C7-C8 region of the spinal cord was less strongly affected, but the C5 and C6 regions showed a massive reduction in the number of immunopositive motor neurons.
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Analysis Tools