Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:85300
Helmbacher F, et al., Met signaling is required for recruitment of motor neurons to PEA3-positive motor pools. Neuron. 2003 Aug 28;39(5):767-77
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:3763237
|
Gene symbol:
|
Isl1
|
Gene name:
|
ISL1 transcription factor, LIM/homeodomain
|
Antibody:
|
clone 39.4D5
|
Assay notes:
|
The authors describe two antibodies that recognize Isl and Isl2; this assay may used another antibody (MGI:1314839).
|
|
Results
|
Specimen
2D:
embryonic day 11.5
(more )
Note: 49 somites. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:3763238).
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
49 somites. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:3763238).
|
Specimen
2E:
embryonic day 11.5; Pax3Sp-d/ Pax3Sp-d
(more )
Note: 49 somites. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:3763238).
Specimen
2E:
(close )
Genetic Background:
|
C57BL/6J-Pax3Sp-d
|
Mutant Allele(s):
|
Pax3Sp-d/Pax3Sp-d
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
49 somites. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:3763238).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
|
2E
|
Expression was unchanged in the mutant spinal cord.
|
Specimen
2F:
embryonic day 11.5; Mettm1Cpo/ Mettm1Cpo
(more )
Note: 49 somites. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:3763238).
Specimen
2F:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
Mutant Allele(s):
|
Mettm1Cpo/Mettm1Cpo
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
49 somites. Double labeled: green - Isl1 and Isl2; red - Etv4 (assay MGI:3763238).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
|
2F
|
Expression was unchanged in the mutant spinal cord.
|
|