Probe:	RATGAD 65/A
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	1a	Expression is entirely nervous system specific.
TS17: nervous system	Present	Regionally restricted	1a	Expression is localized to spatially restricted domains with well-defined boundaries. Signals are predominantly confined to the differentiating zone, with little, if any, labeling in the ventricular zone.
TS17: nasal pit	Present	Regionally restricted	1a	Discrete hybridization signals are seen in the olfactory placode.
TS17: telencephalon	Strong	Regionally restricted	1a	Expression is strong in the medial ganglionic eminence of the ventral telencephalon. These cells form a multilayer structure corresponding to the pallidal differentiating zone.
TS17: diencephalon	Strong	Regionally restricted	1a	Strong expression is detected in the lateral part of the ventral diencephalon. A thin layer of expression is found just above the hypothalamic and ventral thalamic ventricular neuroepithelium. No expression is in ventral and mammillary hypothalamic areas.
TS17: midbrain	Strong	Regionally restricted	1a	There expression in the tectum and strong expression in the lateral part of the anterior and posterior tegmental differentiating zone, whereas it is free of signal ventrally. No expression is detected in the future interior and superior colliculi.
TS17: hindbrain	Strong	Regionally restricted	1a	There is strong expression in the differentiating zone abutting the pontine and medullary ventricular neuroepithelium of the basal plate, as well as in the myencephalic basal plate. No expression is detected in the future cerebellum.
TS17: future spinal cord	Present		1a

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	1b	Expression is entirely nervous system specific.
TS17: nervous system	Present	Regionally restricted	1b	Expression is localized to spatially restricted domains with well-defined boundaries. Signals are predominantly confined to the differentiating zone, with little, if any, labeling in the ventricular zone.
TS17: telencephalon	Strong	Regionally restricted	1b	Expression is strong in the medial ganglionic eminence; these cells form a multilayer structure corresponding to the pallidal differentiating zone. No expression is detected in the future neocortex, hippocampus, rhinencephalon, amygdala, or septal area.
TS17: midbrain	Strong	Regionally restricted	1b	There expression in the tectum and is strong expression in the lateral part of the anterior and posterior tegmental differentiating zone, whereas it is free of signal ventrally. No expression is detected in the future interior and superior colliculi.
TS17: hindbrain	Strong	Regionally restricted	1b	There is strong expression in the myencephalic basal plate. No expression is detected in the future cerebellum.
TS17: future spinal cord	Present		1b
TS17: diencephalon	Present	Regionally restricted	1b	Although there is expression elswhere in the diencephalon, no expression is detected in the dorsal thalamus, epithalamus or in the posterior preoptic area.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	1c	Expression is entirely nervous system specific.
TS17: nervous system	Present	Regionally restricted	1c	Expression is localized to spatially restricted domains with well-defined boundaries. Signals are predominantly confined to the differentiating zone, with little, if any, labeling in the ventricular zone.
TS17: telencephalon	Strong	Regionally restricted	1c	Expression is strong in the medial ganglionic eminence; these cells form a multilayer structure corresponding to the pallidal differentiating zone. No expression is detected in the future neocortex, hippocampus, rhinencephalon, amygdala, or septal area.
TS17: hindbrain	Strong	Regionally restricted	1c	There is strong expression in the myencephalic basal plate. No expression is detected in the future cerebellum.
TS17: future spinal cord	Present		1c
TS17: diencephalon	Present	Regionally restricted	1c	Although there is expression elswhere in the diencephalon, no expression is detected in the dorsal thalamus, epithalamus or in the posterior preoptic area.
TS17: midbrain	Present	Regionally restricted	1c	Although there is expression elswhere in the midbrain, no expression is detected in the future interior and superior colliculi.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Moderate	Regionally restricted	3a	There is strong to moderate expression in a large cluster of densely packed cells in the pallidal differenting zone of the medial ganglionic eminence.
TS19: midbrain	Moderate	Regionally restricted	3a	There is medium to high expression in pretectal and tegmental differentiating zones.
TS19: olfactory epithelium	Present		3a

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Strong	Regionally restricted	3b	There is strong expression in the olfactory epithelium of the ventral aspect of the olfactory pit.
TS19: telencephalon	Moderate	Regionally restricted	3b	There is strong to moderate expression in a large cluster of densely packed cells in the pallidal differenting zone of the medial ganglionic eminence.
TS19: diencephalon	Present	Regionally restricted	3b	There are numerous labeled cells in the anterior hypothalamic differentiating zone.
TS19: midbrain	Moderate	Regionally restricted	3b	There is medium to high expression in pretectal and tegmental differentiating zones.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Moderate	Regionally restricted	3c	There is strong to moderate expression in a large cluster of densely packed cells in the pallidal differenting zone of the medial ganglionic eminence.
TS19: future spinal cord	Present	Regionally restricted	3c	Expression is present in the dorsal and ventral aspects of the differentiating neuroepithelium. It was highest in the intermediate zone and dorsal horn differentiating zone.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	3d	Expression is present in the dorsal and ventral aspects of the differentiating neuroepithelium. It was highest in the intermediate zone and dorsal horn differentiating zone.
TS19: telencephalon	Present	Regionally restricted	3d	Expression is present at the basal midline, close to the septal ventricular neuroepithelium.