Probe:	Clone 1A/1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The probe used in this assay recognizes multiple message isoforms.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	1a'	Expression is entirely nervous system specific.
TS17: nervous system	Present	Regionally restricted	1a'	Expression is localized to spatially restricted domains with well-defined boundaries. Signals are predominantly confined to the differentiating zone, with little, if any, labeling in the ventricular zone.
TS17: telencephalon	Strong	Regionally restricted	1a'	Expression is strong in the medial ganglionic eminence; these cells form a multilayer structure corresponding to the pallidal differentiating zone. No expression is detected in the future neocortex, hippocampus, rhinencephalon, amygdala, or septal area.
TS17: midbrain	Strong	Regionally restricted	1a'	There is expression in the tectum and strong expression in the lateral part of the anterior and posterior tegmental differentiating zone, whereas it is free of signal ventrally. No expression is detected in the future interior and superior colliculi.
TS17: hindbrain	Strong	Regionally restricted	1a'	There is strong expression in the differentiating zone abutting the pontine and medullary ventricular neuroepithelium of the basal plate, as well as in the myencephalic basal plate. No expression is detected in the future cerebellum.
TS17: future spinal cord	Present		1a'
TS17: diencephalon	Present	Regionally restricted	1a'	Although there is expression elswhere in the diencephalon, no expression is detected in the dorsal thalamus, epithalamus or in the posterior preoptic area.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	1b'	Expression is entirely nervous system specific.
TS17: nervous system	Present	Regionally restricted	1b'	Expression is localized to spatially restricted domains with well-defined boundaries. Signals are predominantly confined to the differentiating zone, with little, if any, labeling in the ventricular zone.
TS17: telencephalon	Strong	Regionally restricted	1b'	Expression is strong in the medial ganglionic eminence; these cells form a multilayer structure corresponding to the pallidal differentiating zone. No expression is detected in the future neocortex, hippocampus, rhinencephalon, amygdala, or septal area.
TS17: midbrain	Strong	Regionally restricted	1b'	There is expression in the tectum and strong expression in the lateral part of the anterior and posterior tegmental differentiating zone, whereas it is free of signal ventrally. No expression is detected in the future interior and superior colliculi.
TS17: hindbrain	Strong	Regionally restricted	1b'	There is strong expression in the myencephalic basal plate. No expression is detected in the future cerebellum.
TS17: future spinal cord	Present		1b'
TS17: diencephalon	Present	Regionally restricted	1b'	Although there is expression elswhere in the diencephalon, no expression is detected in the dorsal thalamus, epithalamus or in the posterior preoptic area.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	1c'	Expression is entirely nervous system specific.
TS17: nervous system	Present	Regionally restricted	1c'	Expression is localized to spatially restricted domains with well-defined boundaries. Signals are predominantly confined to the differentiating zone, with little, if any, labeling in the ventricular zone.
TS17: telencephalon	Strong	Regionally restricted	1c'	Expression is strong in the medial ganglionic eminence; these cells form a multilayer structure corresponding to the pallidal differentiating zone. No expression is detected in the future neocortex, hippocampus, rhinencephalon, amygdala, or septal area.
TS17: hindbrain	Strong	Regionally restricted	1c'	There is strong expression in the myencephalic basal plate. No expression is detected in the future cerebellum.
TS17: future spinal cord	Present		1c'
TS17: diencephalon	Present	Regionally restricted	1c'	Although there is expression elswhere in the diencephalon, no expression is detected in the dorsal thalamus, epithalamus or in the posterior preoptic area.
TS17: midbrain	Present	Regionally restricted	1c'	Although there is expression elswhere in the midbrain, no expression is detected in the future interior and superior colliculi.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Moderate	Regionally restricted	3a'	There is strong to moderate expression in a large cluster of densely packed cells in the pallidal differenting zone of the medial ganglionic eminence.
TS19: midbrain	Moderate	Regionally restricted	3a'	There is medium to high expression in pretectal and tegmental differentiating zones.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Moderate	Regionally restricted	3b'	There is strong to moderate expression in a large cluster of densely packed cells in the pallidal differenting zone of the medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present		3c'
TS19: telencephalon	Present	Regionally restricted	3c'	Expression is present at the basal midline, close to the septal ventricular neuroepithelium.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Moderate	Regionally restricted	3d'	There is strong to moderate expression in a large cluster of densely packed cells in the pallidal differenting zone of the medial ganglionic eminence.
TS19: future spinal cord	Present		3d'