Probe:	Fgf-8b cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The probe used in this assay hybridizes to all known isoforms.

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		2A

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		2B

Structure	Level	Pattern	Image	Note
TS15: fronto-nasal process	Present		2C
TS15: 1st branchial arch ectoderm	Present	Regionally restricted	2C	Expression is present in the surface ectoderm at the superior boundary of the mandibular arch and the inferior boundary of the maxillary arch.
TS15: forelimb bud	Absent		2C
TS15: future hindbrain	Present	Regionally restricted	2C	Expression is present in a dorsal to ventral arc at the level of the midbrain-hindbrain junction. This expression is not present in the dorsal midline.
TS15: future midbrain	Present	Regionally restricted	2C	Expression is present in a dorsal to ventral arc at the level of the midbrain-hindbrain junction. This expression is not present in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS15: fronto-nasal process	Present		2D
TS15: 1st branchial arch ectoderm	Present	Regionally restricted	2D	Expression is present in the surface ectoderm at the superior boundary of the mandibular arch and the inferior boundary of the maxillary arch.
TS15: forelimb bud	Absent		2D
TS15: future forebrain	Present		2D
TS15: future midbrain	Weak		2D

Structure	Level	Pattern	Image	Note
TS15: fronto-nasal process	Present		2E
TS15: 1st branchial arch ectoderm	Present	Regionally restricted	2E	Expression is present in the surface ectoderm at the superior boundary of the mandibular arch and the inferior boundary of the maxillary arch.
TS15: future hindbrain	Present	Regionally restricted	2E	Expression is present in a dorsal to ventral arc at the level of the midbrain-hindbrain junction. This expression is not present in the dorsal midline.
TS15: future midbrain	Present	Regionally restricted	2E	Expression is present in a dorsal to ventral arc at the level of the midbrain-hindbrain junction. This expression is not present in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch ectoderm	Present		3B , 3A	Expression is present in the surface ectoderm of the first branchial arches.
TS17: nasal pit	Present		3B , 3A
TS17: head	Present		3A , 3B
TS17: forelimb bud ectoderm	Present		3A , 3B	Expression is present over the entire apical surface.
TS17: hindbrain	Present	Regionally restricted	3B , 3A	Expression is present in the mesencephalon-metencephalon junction.
TS17: midbrain	Present	Regionally restricted	3B , 3A	Expression is present in the mesencephalon-metencephalon junction.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Absent

Structure	Level	Pattern	Image	Note
TS19: nasal pit	Present	Regionally restricted	3C , 3D	Expression is present in the surface ectoderm of the nasal pits.
TS19: 1st branchial arch	Absent		3C , 3D
TS19: head	Present		3D , 3C
TS19: forelimb bud ectoderm	Present		3D , 3C	Expression is present on the entire apical surface.
TS19: hindlimb bud ectoderm	Present		3D , 3C	Expression is present on the entire apical surface.
TS19: metencephalon	Present	Regionally restricted	3D , 3C	Expression is present in the mesencephalon-metencephalon junction.
TS19: midbrain	Present	Regionally restricted	3D , 3C	Expression is present in the mesencephalon-metencephalon junction.
TS19: infundibular recess of 3rd ventricle	Present		3D , 3C	Expression is present in the infundibulum.

Structure	Level	Pattern	Image	Note
TS20: nasal cavity epithelium	Present			Expression is present in the nasal pit epithelia.