Gene Expression Data
Assay Details
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Assay
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Reference:
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J:59927
Guerriero A, et al., PU.1 is required for myeloid-derived but not lymphoid-derived dendritic cells. Blood. 2000 Feb 1;95(3):879-85
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3710171
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Gene symbol:
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Cd24a
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Gene name:
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CD24a antigen
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Antibody:
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Anti-CD24
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Detection system:
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Secondary antibody coupled to alkaline phosphatase
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Assay notes:
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Wild type embryos could have been either PU.1+/+ or PU.1+/-. The results in Fig. 4 were presented in a graph format depicting data from sections (average of 4 embryos) analyzed for relative staining intensity of mutant compared to wild type samples.
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Results
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Specimen
4 E14.5 WT:
embryonic day 14.5
(more  )
Specimen
4 E14.5 WT:
(close  )
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Genetic Background:
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involves: 129S/SvEv
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
4 E14.5 PU.1-/-:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Spi1tm1Sing/Spi1tm1Sing
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: thymus primordium |
Present |
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4_HSA PU.1-/- E14.5
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Expression level in the mutant was equivalent to wild type.
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Specimen
4 E16.5 WT:
embryonic day 16.5
(more )
Specimen
4 E16.5 WT:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thymus |
Present |
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4_HSA WT E16.5
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Specimen
4 E16.5 PU.1-/-:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Spi1tm1Sing/Spi1tm1Sing
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thymus |
Present |
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4_HSA PU.1-/- E16.5
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Expression level in the mutant was equivalent to wild type.
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Analysis Tools
