Probe:	AC1 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	At all times expression appears to be restricted to postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Moderate	Regionally restricted		There is moderate expression in the retinal ganglion cells.
TS23: cortical plate	Moderate	Regionally restricted		There is moderate expression in cortical layers IV, V, and VI.
TS23: telencephalon	Moderate	Non-Uniform		There is moderate expression in hippocampal areas CA1 and CA2 and in the subiculum. There is weak expression in the anterior amygdala, medial septum, and globus pallidus. There is no expression in CA3 and the central medial and basomedial nuclei.
TS23: olfactory lobe	Moderate
TS23: corpus striatum	Moderate
TS23: thalamus	Strong	Non-Uniform		Strong expression in the ventrobasal and posterior nuclei, dorsal lateral geniculate nucleus (LGN) and subthalmus. Weak expression in the ventral LGN and ventrolateral nucleus. Moderate expression in the reticularis. None in the anteroventral nucleus.
TS23: epithalamus	Absent			There is no expression in the habenula.
TS23: hypothalamus	Moderate	Regionally restricted		There is moderate expression in the arcuate nucleus. There is no expression in the hypothalamus proper and the preoptic and supraoptic regions.
TS23: midbrain	Strong	Non-Uniform		There is strong expression in the inferior colliculus, weak expression in the superior colliculus, and no expression in the substantia nigra.
TS23: pons	Strong	Non-Uniform		There is strong expression in the trigeminal and cochlear nuclei. There is moderate expression in the pontine nucleus. There is weak expression in the locus coeruleus.
TS23: medulla oblongata	Moderate	Regionally restricted		There is moderate expression in the inferior olive.
TS23: cerebellum	Present	Regionally restricted		There is moderate expression in the Purkinje cells and deep cerebellar nucleus. There is no expression in the granule cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Strong
TS28: cortical layer I	Weak
TS28: cortical layer II	Weak
TS28: cortical layer III	Weak
TS28: cortical layer IV	Moderate
TS28: cortical layer V	Strong
TS28: cortical layer VI	Strong
TS28: dentate gyrus	Moderate
TS28: hippocampus CA1	Weak
TS28: hippocampus CA2	Moderate
TS28: hippocampus CA3	Absent
TS28: hippocampus	Strong			There is strong expression in the hippocampal interneurons.
TS28: subiculum	Moderate
TS28: main olfactory bulb	Moderate
TS28: amygdala	Strong	Non-Uniform		There is strong expression in the basomedial nucleus and moderate expression in the anterior amygdala.
TS28: central medial thalamic nucleus	Weak
TS28: corpus striatum	Moderate
TS28: medial septal complex	Weak
TS28: globus pallidus	Weak
TS28: basal ventral medial thalamic nucleus	Strong			There is strong expression in the ventrobasal nucleus.
TS28: posterior thalamic nucleus	Moderate			There is moderate expression in the posterior and ventrolateral nuclei.
TS28: ventral posterior lateral thalamic nucleus	Moderate			There is moderate expression in the posterior and ventrolateral nuclei.
TS28: anterior ventral thalamic nucleus	Absent			There is no expression in the anteroventral nucleius.
TS28: lateral geniculate nucleus	Strong	Non-Uniform		There is strong expression in the dorsal lateral geniculate and weak expression ventral lateral geniculate.
TS28: reticular thalamic nucleus	Strong
TS28: subthalamic nucleus	Strong
TS28: habenula	Absent
TS28: hypothalamus	Weak	Non-Uniform		There is weak expression in the hypothalamus. There is no expression in the preoptic and supraoptic areas.
TS28: arcuate nucleus	Moderate
TS28: superior colliculus	Weak
TS28: inferior colliculus	Strong
TS28: substantia nigra	Absent
TS28: locus coeruleus	Weak
TS28: raphe nucleus	Absent
TS28: pontine nucleus	Moderate
TS28: inferior olivary nucleus	Moderate
TS28: trigeminal nucleus	Strong
TS28: cochlear nucleus	Strong
TS28: cerebellum Purkinje cell layer	Strong
TS28: cerebellum granule cell layer	Absent
TS28: deep cerebellar nucleus	Strong

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Strong
TS28: cortical layer I	Weak
TS28: cortical layer II	Weak
TS28: cortical layer III	Strong
TS28: cortical layer IV	Strong
TS28: cortical layer V	Strong
TS28: cortical layer VI	Strong
TS28: dentate gyrus	Strong
TS28: hippocampus CA1	Moderate
TS28: hippocampus CA2	Strong
TS28: hippocampus CA3	Weak
TS28: hippocampus	Strong			There is strong expression in the hippocampal neurons.
TS28: subiculum	Strong
TS28: main olfactory bulb	Weak
TS28: amygdala	Strong			There is strong expression in the amygdala--in the anterior and the basomedial nuclei.
TS28: central medial thalamic nucleus	Strong
TS28: corpus striatum	Moderate
TS28: globus pallidus	Weak
TS28: basal ventral medial thalamic nucleus	Strong			There is strong expression in the ventrobasal nucleus.
TS28: anterior ventral thalamic nucleus	Moderate			There is moderate expression in the posterior, ventrolateral, and anteroventral nuclei.
TS28: posterior thalamic nucleus	Moderate			There is moderate expression in the posterior, ventrolateral, and anteroventral nuclei.
TS28: ventral posterior lateral thalamic nucleus	Moderate			There is moderate expression in the posterior, ventrolateral, and anteroventral nuclei.
TS28: lateral geniculate nucleus	Strong	Non-Uniform		There is strong expression in the dorsal lateral geniculate and weak expression in the ventral lateral geniculate.
TS28: reticular thalamic nucleus	Strong
TS28: subthalamic nucleus	Strong
TS28: habenula	Absent
TS28: hypothalamus	Weak	Non-Uniform		There is weak expression in the hypothalamus. There is no expression in the supraoptic area.
TS28: arcuate nucleus	Absent
TS28: superior colliculus	Weak
TS28: inferior colliculus	Strong
TS28: substantia nigra	Weak
TS28: locus coeruleus	Absent
TS28: raphe nucleus	Absent
TS28: inferior olivary nucleus	Moderate
TS28: pontine nucleus	Moderate
TS28: trigeminal nucleus	Strong
TS28: cochlear nucleus	Strong
TS28: cerebellum Purkinje cell layer	Weak
TS28: cerebellum granule cell layer	Strong
TS28: deep cerebellar nucleus	Moderate

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Weak
TS28: cortical layer II	Weak
TS28: cortical layer III	Weak
TS28: cortical layer IV	Weak
TS28: retina ganglion cell layer	Weak
TS28: cortical layer V	Moderate
TS28: cortical layer VI	Moderate
TS28: dentate gyrus	Strong
TS28: hippocampus CA1	Weak
TS28: hippocampus CA2	Strong
TS28: hippocampus CA3	Absent
TS28: hippocampus	Present	Regionally restricted		Although there is expression elsewhere in the hippocampus, there is no expression in the hippocampal interneurons.
TS28: subiculum	Moderate
TS28: main olfactory bulb	Weak
TS28: amygdala	Absent
TS28: central medial thalamic nucleus	Moderate
TS28: corpus striatum	Absent
TS28: globus pallidus	Absent
TS28: medial septal complex	Absent
TS28: basal ventral medial thalamic nucleus	Weak			There is weak expression in the ventrobasal nucleus.
TS28: anterior ventral thalamic nucleus	Absent			There is no expression in the posterior, ventrolateral, and anteroventral nuclei.
TS28: posterior thalamic nucleus	Absent			There is no expression in the posterior, ventrolateral, and anteroventral nuclei.
TS28: ventral posterior lateral thalamic nucleus	Absent			There is no expression in the posterior, ventrolateral, and anteroventral nuclei.
TS28: lateral geniculate nucleus	Weak	Regionally restricted		There is weak expression in the dorsal lateral geniculate and no expression in the ventral lateral geniculate.
TS28: arcuate nucleus	Absent
TS28: habenula	Absent
TS28: superior colliculus	Absent
TS28: hypothalamus	Absent			There is no expression in the hypothalamus or the preoptic and supraoptic areas.
TS28: reticular thalamic nucleus	Weak
TS28: subthalamic nucleus	Weak
TS28: inferior colliculus	Weak
TS28: inferior olivary nucleus	Absent
TS28: locus coeruleus	Absent
TS28: pontine nucleus	Absent
TS28: raphe nucleus	Absent
TS28: substantia nigra	Absent
TS28: trigeminal nucleus	Weak
TS28: cochlear nucleus	Weak
TS28: cerebellum Purkinje cell layer	Absent
TS28: deep cerebellar nucleus	Absent
TS28: cerebellum granule cell layer	Strong

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present		1A	Expression is distributed over all the cortical layers.
TS28: cortical layer V	Present		1A	This is one of the layer's with the most intense expression.
TS28: cortical layer VI	Present		1A	This is one of the layer's with the most intense expression.
TS28: lateral geniculate nucleus	Present		1A	Expression is present in the dorsal and medial lateral geniculate.
TS28: basal ventral medial thalamic nucleus	Present		1A	Expression is present in the sensory thalamic relays, the ventrobasal nucleus, and the posterior nucleus.
TS28: dorsal thalamus	Present		1A	Expression is present in the sensory thalamic relays, the ventrobasal nucleus, and the posterior nucleus.
TS28: posterior thalamic nucleus	Present		1A	Expression is present in the sensory thalamic relays, the ventrobasal nucleus, and the posterior nucleus.
TS28: dentate gyrus	Strong		1A	The expression is present in the granule neurons and the molecular layer.
TS28: dentate gyrus hilus	Present		1A
TS28: hippocampus stratum oriens	Present		1A
TS28: reticular thalamic nucleus	Present		1A
TS28: subiculum	Present		1A
TS28: hippocampus CA1	Moderate		1A
TS28: hippocampus CA2	Strong		1A

Structure	Level	Pattern	Image	Note
TS28: lateral geniculate nucleus	Present		1B	Expression is present in the dorsal and medial lateral geniculate.
TS28: basal ventral medial thalamic nucleus	Present		1B	Expression is present in the sensory thalamic relays, the ventrobasal nucleus, and the posterior nucleus.
TS28: dorsal thalamus	Present		1B	Expression is present in the sensory thalamic relays, the ventrobasal nucleus, and the posterior nucleus.
TS28: posterior thalamic nucleus	Present		1B	Expression is present in the sensory thalamic relays, the ventrobasal nucleus, and the posterior nucleus.
TS28: dentate gyrus	Strong		1B
TS28: dentate gyrus hilus	Present		1B
TS28: hippocampus stratum oriens	Present		1B
TS28: reticular thalamic nucleus	Present		1B
TS28: subiculum	Present		1B
TS28: hippocampus CA1	Moderate		1B
TS28: hippocampus CA2	Strong		1B

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		1C
TS28: hippocampus CA2	Strong		1C
TS28: hippocampus molecular layer	Present		1C
TS28: hippocampus CA1	Moderate		1C
TS28: cerebral cortex	Present	Non-Uniform	1C	Expression is broadly distributed, with somewhat more abundant expression in the deep cortical laminae (V and VI).
TS28: subiculum	Present		1C
TS28: dorsal thalamus	Trace		1C	There is trace expression in the dorsal thalamic nuclei.

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Strong		2A	Expression is present in the deep cerebellar nuclei.
TS23: pons	Strong	Regionally restricted	2A	Strong expression is present in the cochlear and trigeminal nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		2B
TS28: cochlear nucleus	Present		2B
TS28: deep cerebellar nucleus	Present		2B
TS28: cerebellum granule cell layer	Absent		2B
TS28: trigeminal nucleus	Present		2B
TS28: superior colliculus	Moderate		2B
TS28: inferior colliculus	Strong		2B

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		2C
TS28: deep cerebellar nucleus	Present		2C
TS28: cerebellum Purkinje cell layer	Present		2C	Expression levels are lower than what was seen earlier.
TS28: cochlear nucleus	Present		2C	Expression levels are lower than what was seen earlier.
TS28: inferior colliculus	Present		2C	Expression levels are lower than what was seen earlier.
TS28: trigeminal nucleus	Present		2C	Expression levels in the trigeminal nucleus are lower than what was seen earlier.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		2D	Expression is essentially limited to the granule cell layer.
TS28: cochlear nucleus	Present		2D
TS28: trigeminal nucleus	Present		2D

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong	Non-Uniform	3A	There is intense labeling seen in all the cortical plate, with more intense labeling in the superficial and deep parts of the plate.
TS23: cerebral cortex subventricular zone	Absent		3A
TS23: cerebral cortex ventricular layer	Absent		3A
TS23: retina nuclear layer	Present		3A	Expression is present in the retinal ganglion cells.
TS23: olfactory lobe	Present	Regionally restricted	3A	Expression is present in the glomerular and mitral layers. It is not present in the ventricular zone.
TS23: olfactory epithelium	Absent		3A

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong	Non-Uniform	3B	There is intense labeling seen in all the cortical plate, with more intense labeling in the superficial and deep parts of the plate.
TS23: cerebral cortex subventricular zone	Absent		3B
TS23: cerebral cortex ventricular layer	Absent		3B
TS23: corpus striatum	Present	Regionally restricted	3B	Expression is present in lateral parts.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong	Non-Uniform	3C	There is intense labeling seen in all the cortical plate, with more intense labeling in the superficial and deep parts of the plate.
TS23: cerebral cortex subventricular zone	Absent		3C
TS23: cerebral cortex ventricular layer	Absent		3C
TS23: telencephalon	Present	Regionally restricted	3C	Expression is present in the hippocampus.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong	Non-Uniform	4A	There is intense labeling seen in all the cortical plate, with more intense labeling in the superficial and deep parts of the plate.
TS23: cerebral cortex subventricular zone	Absent		4A
TS23: cerebral cortex ventricular layer	Absent		4A
TS23: thalamus	Present		4A	Expression is present in the dorsal and ventral thalamus.
TS23: epithalamus	Absent		4A	There is no expression in the habenula or the pineal gland.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong	Non-Uniform	4D	There is intense labeling seen in all the cortical plate, with more intense labeling in the superficial and deep parts of the plate.
TS23: cerebral cortex subventricular zone	Absent		4D
TS23: cerebral cortex ventricular layer	Absent		4D
TS23: thalamus	Present		4D	Expression is present in the dorsal and ventral thalamus.
TS23: epithalamus	Absent		4D	There is no expression in the habenula or the pineal gland.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong	Non-Uniform	4G	There is intense labeling seen in all the cortical plate, with more intense labeling in the superficial and deep parts of the plate.
TS23: cerebral cortex subventricular zone	Absent		4G
TS23: cerebral cortex ventricular layer	Absent		4G
TS23: cerebellum	Present	Regionally restricted	4G	Expression is present in presumptive Purkinje cells and the deep cerebellar nuclei.