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Gene Expression Data
Assay Details
Assay
Reference: J:113442 Winger Q, et al., Analysis of transcription factor AP-2 expression and function during mouse preimplantation development. Biol Reprod. 2006 Sep;75(3):324-33
Assay type: RT-PCR
MGI Accession ID: MGI:3689861
Gene symbol: Tfap2c
Gene name: transcription factor AP-2, gamma
Probe: AP-2-gamma pG, AP-2-gamma pH
Visualized with: Ethidium bromide
Assay notes: Nested primers were used for this experiment. The outer set was MGI:3689827. This experiment took advantage of a polymorphism that occurs between the FVB/NJ and C57BL/6J strains to determine whether the expression observed in two-cell embryos was due to maternally inherited transcripts or due to zygotic transcription.
Results Image: 1C left
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
M Mol. Wt. Marker Lane
FVB-AvaII E1.5 (a) TS2: 2-cell stage conceptus Present Not Specified FVB/NJ Not Specified
FVB-AlwNI E1.5 (a) TS2: 2-cell stage conceptus Present (b) Not Specified FVB/NJ Not Specified
FVBxC57-AvaII E1.5 (a) TS2: 2-cell stage conceptus Present (c) Not Specified (FVB/NJ x C57BL/6J)F1 Not Specified
FVBxC57-AlwNI E1.5 (a) TS2: 2-cell stage conceptus Present Not Specified (FVB/NJ x C57BL/6J)F1 Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The presence of a 137 bp digested product in this lane indicates that maternally inherited RNA is still present at this age.
(c) The presence of a 92 bp digested product in this lane indicates that zygotic transcription from the paternal allele has begun.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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