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Gene Expression Data
Assay Details
Assay
Reference: J:99326 Kuhnert F, et al., Dosage-dependent requirement for mouse Vezf1 in vascular system development. Dev Biol. 2005 Jul 1;283(1):140-156
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3663446
Gene symbol: Tjp1
Gene name: tight junction protein 1
Antibody: Anti-ZO-1
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 6A,B Hemorrhaged: embryonic day 11.5; Vezf1tm1Stmn/Vezf1tm1Stmn (more )
Note: Double labeled: red - Tjp1; green - Pecam1 (assay MGI:3663448). Fig. 6B is a higher magnification image of 6A.
Structure Level Pattern Image Note
TS19: blood vessel Weak Regionally restricted 6B Merged Hemorrhaged , 6B ZO-1 Hemorrhaged , 6A ZO-1 Hemorrhaged , 6A Merged Hemorrhaged Expression was reduced and had a disrupted pattern in the mutant endothelial wall.
Specimen 6A,B Control: embryonic day 11.5 (more )
Note: Double labeled: red - Tjp1; green - Pecam1 (assay MGI:3663448). Fig. 6B is a higher magnification image of 6A.
Structure Level Pattern Image Note
TS19: blood vessel Present Regionally restricted 6A Merged Control , 6A ZO-1 Control , 6B Merged Control , 6B ZO-1 Control Expression was detected in the endothelial wall.
Specimen 7A KO: embryonic day 10.5; Vezf1tm1Stmn/Vezf1tm1Stmn (more )
Note: Double labeled: red - Tjp1; green - Pecam1 (assay MGI:3663448).
Structure Level Pattern Image Note
TS17: blood vessel Present Patchy 7A Zoom KO , 7A merged KO , 7A ZO-1 KO Expression was spotty and discontinuous in vessels and capillaries of the mutant.
Specimen 7A WT: embryonic day 10.5 (more )
Note: Double labeled: red - Tjp1; green - Pecam1 (assay MGI:3663448).
Structure Level Pattern Image Note
TS17: blood vessel Present 7A ZO-1 WT , 7A Zoom WT , 7A merged WT
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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