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Gene Expression Data
Assay Details
Assay
Reference: J:99326 Kuhnert F, et al., Dosage-dependent requirement for mouse Vezf1 in vascular system development. Dev Biol. 2005 Jul 1;283(1):140-156
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3663448
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6A,B Hemorrhaged: embryonic day 11.5; Vezf1tm1Stmn/Vezf1tm1Stmn (more )
Note: Double labeled: green - Pecam1; red - Tjp1 (assay MGI:3663446). Fig. 6B is a higher magnification image of 6A.
Structure Level Pattern Image Note
TS19: blood vessel Present 6A PECAM Hemorrhaged , 6B Merged Hemorrhaged , 6B PECAM Hemorrhaged , 6A Merged Hemorrhaged Expression was detected as in wild type.
Specimen 6A,B Control: embryonic day 11.5 (more )
Note: Double labeled: green - Pecam1; red - Tjp1 (assay MGI:3663446). Fig. 6B is a higher magnification image of 6A.
Structure Level Pattern Image Note
TS19: blood vessel Present 6B PECAM Control , 6B Merged Control , 6A Merged Control , 6A PECAM Control
Specimen 6D Hemorrhaged: embryonic day 11.5; Vezf1tm1Stmn/Vezf1tm1Stmn (more )
Note: Double labeled: green - Pecam1; red - collagen IV.
Structure Level Pattern Image Note
TS19: blood vessel Present 6D Merged Hemorrhaged , 6D PECAM Hemorrhaged Expression was detected as in wild type.
Specimen 6D Control: embryonic day 11.5 (more )
Note: Double labeled: green - Pecam1; red - collagen IV.
Structure Level Pattern Image Note
TS19: blood vessel Present 6D PECAM Control , 6D Merged Control
Specimen 7A KO: embryonic day 10.5; Vezf1tm1Stmn/Vezf1tm1Stmn (more )
Note: Double labeled: green - Pecam1; red - Tjp1 (assay MGI:3663446)
Structure Level Pattern Image Note
TS17: blood vessel Present 7A merged KO , 7A Zoom KO , 7A PECAM KO Expression was detected as in wild type.
Specimen 7A WT: embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Tjp1 (assay MGI:3663446)
Structure Level Pattern Image Note
TS17: blood vessel Present 7A Zoom WT , 7A merged WT , 7A PECAM WT
Specimen 7B KO: embryonic day 10.5; Vezf1tm1Stmn/Vezf1tm1Stmn (more )
Note: Double labeled: green - Pecam1; red - collagen IV.
Structure Level Pattern Image Note
TS17: blood vessel Present 7B merged KO , 7B PECAM KO , 7B Zoom KO Expression was detected as in wild type.
Specimen 7B WT: embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - collagen IV.
Structure Level Pattern Image Note
TS17: blood vessel Present 7B Zoom WT , 7B PECAM WT , 7B merged WT
Specimen 10K: embryonic day 13.5; Vezf1tm1Stmn/Vezf1+ (more )
Note: Double labeled: green - Pecam1; red - Prox1 (assay MGI:3663464).
Structure Level Pattern Image Note
TS21: embryo Present 10K
Specimen 10M: embryonic day 13.5 (more )
Note: Double labeled: green - Pecam1; red - Prox1 (assay MGI:3663464).
Structure Level Pattern Image Note
TS21: embryo Present 10M
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory