Gene Expression Data
Assay Details
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Assay
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Reference:
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J:84605
Jeong Y, et al., Distinct regulators of Shh transcription in the floor plate and notochord indicate separate origins for these tissues in the mouse node. Development. 2003 Aug;130(16):3891-902
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3655953
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Gene symbol:
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Ptch1
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Gene name:
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patched 1
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Reporter:
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lacZ
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Probe:
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lacZ probe
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Probe preparation:
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labelled with digoxigenin RNA
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Results
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Specimen
7F:
embryonic day 8.0; Ptch1tm1Mps/Ptch1 +
(more  )
Note: 5-6 somite stage.
Specimen
7F:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptch1tm1Mps/Ptch1+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5-6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
7F
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Expression was detected in the dorsal layer of the node and ventral midline.
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Specimen
7F (i):
embryonic day 8.0; Ptch1tm1Mps/Ptch1 +
(more )
Note: 5-6 somite stage. Embedded in 4% agarose. Section at the level of the node.
Specimen
7F (i):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptch1tm1Mps/Ptch1+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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5-6 somite stage. Embedded in 4% agarose. Section at the level of the node.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube floor plate |
Present |
Regionally restricted |
7Fi
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Expression in dorsal and ventral layers of the node and in the presumptive floorplate.
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| TS12: primitive streak |
Present |
Regionally restricted |
7Fi
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Expression in dorsal and ventral layers of the node and in the presumptive floorplate.
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Specimen
7F (ii):
embryonic day 8.0; Ptch1tm1Mps/Ptch1 +
(more )
Note: 5-6 somite stage. Embedded in 4% agarose. Section at level of second most newly formed somite.
Specimen
7F (ii):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ptch1tm1Mps/Ptch1+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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5-6 somite stage. Embedded in 4% agarose. Section at level of second most newly formed somite.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube floor plate |
Present |
Regionally restricted |
7Fii
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Expression in the presumptive floor plate and notochord.
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| TS12: notochord |
Present |
Regionally restricted |
7Fii
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Expression in the presumptive floor plate and notochord.
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Analysis Tools