Probe:	Gad1 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS14: tail bud	Strong		1A

Structure	Level	Pattern	Image	Note
TS17: tail	Present	Regionally restricted	1B	Expression is localized towards the tip of the tail.

Structure	Level	Pattern	Image	Note
TS19: tail	Present	Regionally restricted	1C	Expression is localized towards the tip of the tail.

Structure	Level	Pattern	Image	Note
TS20: tail	Present		1D	Expression is fading.

Structure	Level	Pattern	Image	Note
TS15: tail paraxial mesenchyme	Present		1E	The highest expression levels are localized ventrally, adjacent to the notochord.
TS15: tail neural tube	Present	Regionally restricted	1E	Expression is present in the ventral neural tube
TS15: notochord	Present		1E
TS15: hindgut	Present	Regionally restricted	1E	Expression is present in the cells of the dorsal hindgut.

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Strong	Regionally restricted	1F	Expression is present throughout the mesenchyme in the caudal portion of the tailbud.
TS15: tail future spinal cord	Strong	Regionally restricted	1F	Expression is present in the neural epithelium in the caudal portion of the tailbud.
TS15: tail surface ectoderm	Absent		1F

Structure	Level	Pattern	Image	Note
TS21: tail	Absent

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial pouch	Present		2A	Expression is detected in and around the second, third, and fourth pharyngeal pouches.
TS15: 3rd branchial pouch	Present		2A	Expression is detected in and around the second, third, and fourth pharyngeal pouches.
TS15: branchial pouch	Present		2A	Expression is detected in and around the second, third, and fourth pharyngeal pouches.
TS15: 3rd branchial pouch endoderm	Present		2A , 2B	There is expression in the third pouch endoderm. It is particularly strong in the dorsal portion of the pouch.
TS15: branchial arch mesenchyme	Present		2B , 2A	There is diffuse expression in the mesenchyme caudal to the third pharyngeal pouch.

Structure	Level	Pattern	Image	Note
TS15: 3rd branchial pouch endoderm	Present			There is expression in the third pouch endoderm. It is particularly strong in the dorsal portion of the pouch.

Structure	Level	Pattern	Image	Note
TS14: branchial arch	Weak			There is faint expression in the pharyngeal region.
TS14: forelimb bud	Present

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Absent			Expression is not detectable in the pharyngeal region.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Present		3A , 3B	Expression is present in the pre-apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	3D , 3C	Expression is present in a diffuse stripe in the proximal forelimb.
TS17: forelimb bud apical ectodermal ridge	Present		3D , 3C

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud apical ectodermal ridge	Present	Regionally restricted	3E	Expression is restricted to the apical ectoderm.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	3F , 3G	Expression is present in a diffuse stripe proximal in the proximal forelimb; the expression in the apical ectodermal ridge is fading.
TS19: forelimb bud ectoderm	Strong		3G
TS19: forelimb bud mesenchyme	Strong		3G
TS19: hindlimb bud	Present	Regionally restricted	3H , 3F	Expression is present in a diffuse crescent shape in the proximal hindlimb.

Structure	Level	Pattern	Image	Note
TS20: vibrissa	Present		4B , 4A	Expression is present in the supra-orbital, infra-orbital, and post-oral vibrissae, and in the lateral nasal and maxillary rows, as well as in some of the posterior labial vibrissae.

Structure	Level	Pattern	Image	Note
TS21: vibrissa	Present		4C , 4D	Expression is detected in the anterior lateral nasal and maxillary rows and was activated in the rhinal, labial, and submental vibrissae.

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Strong		4F , 4E	Strong expression is found in the labial, submental, and rhinal vibrissae.

Structure	Level	Pattern	Image	Note
TS20: vibrissa epithelium	Present		4G	Expression is localized to the epidermal placodes of the mystacial vibrisse and is maintained as the placodes begin to invaginate.

Structure	Level	Pattern	Image	Note
TS20: vibrissa epithelium	Present		4H	Expression is localized to the epidermal placodes of the mystacial vibrisse.