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Gene Expression Data
Assay Details
Assay
Reference: J:86170 Menke DB, et al., Sexual differentiation of germ cells in XX mouse gonads occurs in an anterior-to-posterior wave. Dev Biol. 2003 Oct 15;262(2):303-12
Assay type: RNA in situ
MGI Accession ID: MGI:3652407
Gene symbol: Fst
Gene name: follistatin
Probe: Follistatin cDNA1
Probe preparation: labelled with digoxigenin RNA
Results
Specimen 5A: embryonic day 11.5 (more )
Note: 16-17 tail somites.
Structure Level Pattern Image Note
TS19: gonad Present 5A

Specimen 5B: embryonic day 11.5 (more )
Note: 18-19 tail somites.
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 5B Expression was detected scattered throughout most of the gonad, but the anterior and posterior tips were devoid of signal.

Specimen 5C: embryonic day 11.5 (more )
Note: 20-21 tail somites.
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 5C Expression was detected scattered throughout most of the gonad, but the anterior and posterior tips were devoid of signal.

Specimen 5D: embryonic day 12.0 (more )
Note: 22-23 tail somites.
Structure Level Pattern Image Note
TS20: ovary Present Regionally restricted 5D Expression in the genital ridge had spread to the anterior and posterior-most regions.

Specimen 5E: embryonic day 12.0 (more )
Note: 24 tail somites.
Structure Level Pattern Image Note
TS20: ovary Present Regionally restricted 5E Expression in the genital ridge had spread to the anterior and posterior-most regions.

Specimen 5F: embryonic day 11.5 (more )
Note: 16-17 tail somites.
Structure Level Pattern Image Note
TS19: gonad Present 5F

Specimen 5G: embryonic day 11.5 (more )
Note: 18-19 tail somites.
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 5G Expression was detected scattered throughout most of the gonad, but the anterior and posterior tips were devoid of signal. Expression was decreased in the XY gonad compared to the XX gonad.

Specimen 5H: embryonic day 11.5 (more )
Note: 20-21 tail somites.
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 5H Expression was prominent in the anterior and posterior poles of XY genital ridges and was completely absent in central regions.

Specimen 5I: embryonic day 12.0 (more )
Note: 22-23 tail somites.
Structure Level Pattern Image Note
TS20: testis Present Regionally restricted 5I Expression was lost in the anterior pole but was detected in the posterior pole of XY genital ridges.

Specimen 5J: embryonic day 12.0 (more )
Note: 24 tail somites.
Structure Level Pattern Image Note
TS20: testis Present Regionally restricted 5J Expression was detected in the posterior pole.

Specimen 6G: embryonic day 14.5 (more )
Note: XYZal Mildly feminized.
Structure Level Pattern Image Note
TS22: gonad Present Regionally restricted 6G Expression was detected in the feminized regions but was excluded from masculinized regions of mildly feminized ovotestes. Expression levels were variable.

Specimen 6H: embryonic day 14.5 (more )
Note: XYZal Severely feminized.
Structure Level Pattern Image Note
TS22: gonad Present Regionally restricted 6H Expression was strong in the feminized regions but was excluded from masculinized regions of severely feminized ovotestes.

Specimen 6I: embryonic day 14.5 (more )
Note: XYZal Sex reversed.
Structure Level Pattern Image Note
TS22: gonad Present Regionally restricted 6I Expression was detected in the feminized regions but was excluded from masculinized regions of fully feminized ovotestes.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory