Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79830
Garel S, et al., The early topography of thalamocortical projections is shifted in Ebf1 and Dlx1/2 mutant mice. Development. 2002 Dec;129(24):5621-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3629736
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Gene symbol:
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Sema6a
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Gene name:
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sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
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Probe:
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Sema6a probe1
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Probe preparation:
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Antisense RNA
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Assay notes:
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Because they have a have a wild type phenotype, heterozygous animals were used for controls.
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Results
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Specimen
5M:
embryonic day 16.5; Ebf1tm1Rug/Ebf1 +
(more  )
Specimen
5M:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thalamus |
Present |
Regionally restricted |
5M
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Expression is in the dorsal thalamus, low in medial nuclei, and is absent in the dorsal lateral geniculate nucleus.
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Specimen
5N:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1tm1Rug
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thalamus |
Present |
Regionally restricted |
5N
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
6I:
embryonic day 13.5
(more )
Specimen
6I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
6J:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1tm1Rug
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
6K:
embryonic day 15.5
(more )
Specimen
6K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
6L:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1tm1Rug
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: thalamus |
Present |
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6L
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Expression in this tissue is the same as in wild-type embryos.
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| TS23: telencephalon |
Weak |
Regionally restricted |
6L
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Expression is reduced in the basal ganglia.
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Specimen
Not shown, WT:
embryonic day 14.5; Ebf1tm1Rug/Ebf1 +
(more )
Specimen
Not shown, WT:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
Not shown, -/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1tm1Rug
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: thalamus |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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Analysis Tools
