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Gene Expression Data
Assay Details
Assay
Reference: J:3969 Zhou X, et al., Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation. Nature. 1993 Feb 11;361(6412):543-7
Assay type: RNA in situ
MGI Accession ID: MGI:3589355
Gene symbol: Nodal
Gene name: nodal growth differentiation factor
Probe: Nodal cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4a left: embryonic day 7.5 (more )
Note: This embryo is at head fold stage with no visible somites. This view is of the ventral surface with the posterior end up.
Structure Level Pattern Image Note
TS11: node Present 4a left Expression is seen as a ring of staining around the node.
TS11: primitive streak Present Regionally restricted 4a left No expression in the posterior primitive streak
TS11: neural ectoderm Absent 4a left No expression in the neural folds at the anterior end of the embryo.
TS11: allantois Absent 4a left
Specimen 4a middle: embryonic day 7.5 (more )
Note: This embryo is at head fold stage with no visible somites.
Structure Level Pattern Image Note
TS11: node Present 4a middle Expression is seen as a ring of staining around the node.
TS11: allantois Absent 4a middle
Specimen 4a right - left embryo: embryonic day 7.0 (more )
Note: This embryo is at later primitive streak stage but contains a node.
Structure Level Pattern Image Note
TS10: node Present 4a right - left embryo
Specimen 4a right - right embryo: embryonic day 7.0 (more )
Note: This embryo is at later primitive streak stage but has no node.
Structure Level Pattern Image Note
TS10: embryo Weak 4a right - right embryo There is a very low level of expression and no discrete node.
Specimen 4b left: embryonic day 8.0 (more )
Note: This embryo is at head fold stage. This is a parasagittal section.
Structure Level Pattern Image Note
TS11: node Present Regionally restricted 4b left Expression is present in the node in a small group of external cells (possibly endoderm) and cells immediately subadjacent to them.
Specimen 4b right: embryonic day 8.0 (more )
Note: This embryo is at head fold stage. This is a parasagittal section.
Structure Level Pattern Image Note
TS11: node Present Regionally restricted 4b right Expression is present in the node in a small group of external cells (possibly endoderm) and cells immediately subadjacent to them.
Specimen Not shown: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS12: embryo Absent Lack of expression coincides with disappearance of the node as a distinct structure.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory