Probe:	UI-M-AO1-aej-g-05-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	This data is from Supplemental Table 5, Gene column, row: 12775 pyruvate dehydrogenase kinase 3. After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde prior to processing.

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted	S4_Pdk3 E14	Expression was detected in the outer neuroblastic layer but was higher in a subset of cells.

Structure	Level	Pattern	Image	Note
TS24: retina nuclear layer	Present	Regionally restricted	S4_Pdk3 E16	Expression was detected in the outer neuroblastic layer but was higher in a subset of cells.

Structure	Level	Pattern	Image	Note
TS26: retina outer nuclear layer	Present		S4_Pdk3 E18	Expression was detected in the outer neuroblastic layer but was higher in a subset of cells.

Structure	Level	Pattern	Image	Note
TS27: retina outer nuclear layer	Present		S4_Pdk3 P0	Expression was detected in the outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS27: retina outer nuclear layer	Present		S4_Pdk3 P2	Expression was detected in the outer neuroblastic layer but was higher in the vitreal outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present	Regionally restricted	S4_Pdk3 P4	Expression was weak in the outer neuroblastic layer but was higher in the vitreal outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	S4_Pdk3 P6	Expression was detected in the Muller glia.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted		Expression was detected in the Muller glia.

Structure	Level	Pattern	Image	Note
TS28: retina	Absent		S4_Pdk3 Adult

Structure	Level	Pattern	Image	Note
TS28: central nervous system	Present			Expression was widespread.

Structure	Level	Pattern	Image	Note
TS24: embryo	Present			Expression was widespread.