Gene Expression Data
Assay Details
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Assay
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Reference:
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J:82351
Bondjers C, et al., Transcription profiling of platelet-derived growth factor-B-deficient mouse embryos identifies RGS5 as a novel marker for pericytes and vascular smooth muscle cells. Am J Pathol. 2003 Mar;162(3):721-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3581537
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Gene symbol:
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Rgs5
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Gene name:
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regulator of G-protein signaling 5
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Probe:
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RGS5 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 12.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: blood vessel |
Present |
Regionally restricted |
1A
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Expression is present in the microvessels of the hindbrain; other staining indicates that these cells are pericytes.
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Specimen
1B:
embryonic day 12.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: blood vessel |
Present |
Regionally restricted |
1B
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Expression is present in the microvessels of the hindbrain; other staining indicates that these cells are pericytes.
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Specimen
1C:
embryonic day 14.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Present |
Regionally restricted |
1C
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Expression is present in the microvessels of the hindbrain; other staining indicates that these cells are pericytes.
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Specimen
1D:
embryonic day 14.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Present |
Regionally restricted |
1D
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Expression is present in the microvessels of the hindbrain; other staining indicates that these cells are pericytes.
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Specimen
2A:
embryonic day 14.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Present |
Regionally restricted |
2A
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In the brain, expression is present in the pericytes surrounding the capillaries.
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Specimen
2D:
embryonic day 14.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: arterial system |
Present |
Regionally restricted |
2D
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Expression is present in the vascular smooth muscle cells surrounding some of the larger arteries.
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| TS22: venous system |
Absent |
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2D
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There is no, or limited, expression in the veins.
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Specimen
2G:
embryonic day 14.5
(more )
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: renal cortex |
Present |
Regionally restricted |
2G
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Expression is present in the mesangial cells in the kidney.
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Specimen
2J:
embryonic day 14.5
(more )
Specimen
2J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung mesenchyme |
Present |
Regionally restricted |
2J
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Expression is present in the mesenchyme around developing airways. There is also weak expression in the vascular smooth muscle cells surrounding developing lung arterial branches.
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Specimen
2M:
embryonic day 14.5
(more )
Specimen
2M:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: gut |
Present |
Regionally restricted |
2M
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Expression is present in the gut wall at the site of the developing smooth muscle cell layers. It is not present in the inner mesenchymal layer.
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Specimen
3A:
embryonic day 14.5; Pdgfbtm1Cbet/Pdgfb +
(more )
Specimen
3A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Pdgfbtm1Cbet/Pdgfb+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
Regionally restricted |
3A
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Expression is present in pericytes in the brain.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Pdgfbtm1Cbet/Pdgfbtm1Cbet
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brain |
Present |
Regionally restricted |
3B
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Expression is present in pericytes in the brain; a few of these persist in the mutant.
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Specimen
3G:
embryonic day 14.5; Pdgfbtm1Cbet/Pdgfb +
(more )
Note: Double labeled with isolectin (brown).
Specimen
3G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Pdgfbtm1Cbet/Pdgfb+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled with isolectin (brown).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Present |
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3G
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Expression is present in the pericytes located in the capillary walls in the brain.
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Pdgfbtm1Cbet/Pdgfbtm1Cbet
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled with isolectin (brown).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Present |
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3H
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Expression is present in the pericytes located in the capillary walls in the brain; there are fewer of these cells in the mutant comopared to wild type.
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Specimen
3I:
embryonic day 14.5; Pdgfbtm1Cbet/Pdgfb +
(more )
Note: Double labeled with isolectin (brown).
Specimen
3I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Pdgfbtm1Cbet/Pdgfb+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled with isolectin (brown).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: arterial system |
Present |
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3I
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Expression is present in developing arteries.
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Specimen
3J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Pdgfbtm1Cbet/Pdgfbtm1Cbet
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled with isolectin (brown).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: arterial system |
Present |
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3J
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In the mutant, there is residual expression in the developing arteries.
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