Pdgfb^tm1Cbet
Targeted Allele Detail

Summary

• Symbol: Pdgfb^tm1Cbet
• Name: platelet derived growth factor, B polypeptide; targeted mutation 1, Christer Betsholtz
• MGI ID: MGI:1933334
• Synonyms: PDGF-B-
• Gene: Pdgfb Location: Chr15:79880075-79899178 bp, - strand Genetic Position: Chr15, 37.85 cM, cytoband E
• Alliance: Pdgfb^tm1Cbet page

Abnormal endothelial cell morphology and increased caveolae formation in Pdgfb^tm1Cbet/Pdgfb^tm1Cbet brains

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:20017
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin cassette replaced part of exon 3 and all of exon 4. The targeting event is predicted to prevent the formation of a major part of the protein, including cysteine residues involved in all inter- and intramolecular sulfide bridges. (J:20017)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:	42 assay results
In Structures Affected by this Mutation:	19 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfb Mutation: 15 strains or lines available

Notes

Mutants in J:89186 provide a model for microvascular changes that bear striking resemblance to diabetic microangiopathy.

References

• Original: J:20017 Leveen P, et al., Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities. Genes Dev. 1994 Aug 15;8(16):1875-87
• All: 24 reference(s)