Gene Expression Data
Assay Details
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Assay
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Reference:
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J:94054
Sakai Y, et al., Co-expression of de novo DNA methyltransferases Dnmt3a2 and Dnmt3L in gonocytes of mouse embryos. Gene Expr Patterns. 2004 Dec;5(2):231-7
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:3528809
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Gene symbol:
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Dnmt3b
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Gene name:
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DNA methyltransferase 3B
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Probe:
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Dnmt3b-pC, Dnmt3b-pD
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Probe preparation:
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labelled with SYBR green
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Assay notes:
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The primer set designed for the 3' region of Dnmt3b amplifies 332 and 143-bp fragments for the Dnmt3b1/Dnmt3b2 and Dnmt3b3/Dnmt3b6 transcripts,
respectively. The gel was stained with SYBR Green I and scanned on a fluoroimager.
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Results
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| E14.0
(a)
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TS22: testis
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Present
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Trace
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Not Specified; total RNA |
Not Specified |
| Male |
| E16.0
(a)
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TS24: testis
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Strong
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Trace
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Not Specified; total RNA |
Not Specified |
| Male |
| E18.0
(a)
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TS26: testis
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Present
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Trace
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Not Specified; total RNA |
Not Specified |
| Male |
| P2
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TS27: testis
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Strong
(b)
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Trace
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Not Specified; total RNA |
Not Specified |
| Male |
| P4
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TS28: testis
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Strong
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Trace
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Not Specified; total RNA |
Not Specified |
| Male |
| P6
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TS28: testis
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Strong
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Weak
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Not Specified; total RNA |
Not Specified |
| Male |
| Mol. Wt. Marker Lane |
Notes:
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Analysis Tools
