Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:94054
Sakai Y, et al., Co-expression of de novo DNA methyltransferases Dnmt3a2 and Dnmt3L in gonocytes of mouse embryos. Gene Expr Patterns. 2004 Dec;5(2):231-7
|
|
Assay type:
|
RT-PCR
|
|
MGI Accession ID:
|
MGI:3528723
|
|
Gene symbol:
|
Dnmt3b
|
|
Gene name:
|
DNA methyltransferase 3B
|
|
Probe:
|
Dnmt3b-pA, Dnmt3b-pB
|
|
Probe preparation:
|
labelled with SYBR green
|
|
Assay notes:
|
The primer set designed for the 5' region of Dnmt3b amplifies 296 and 236-bp fragments derived from the Dnmt3b1/Dnmt3b6 and Dnmt3b2/Dnmt3b3 transcripts, respectively. The gel was stained with SYBR Green I and scanned on a fluoroimager.
|
|
|
Results
|
| E14.0
(a)
|
TS22: testis
|
Trace
|
Strong
|
Not Specified; total RNA |
Not Specified |
| Male |
| E16.0
(a)
|
TS24: testis
|
Trace
|
Very strong
|
Not Specified; total RNA |
Not Specified |
| Male |
| E18.0
(a)
|
TS26: testis
|
Trace
|
Strong
|
Not Specified; total RNA |
Not Specified |
| Male |
| P2
|
TS27: testis
|
Weak
|
Present
|
Not Specified; total RNA |
Not Specified |
| Male |
| P4
|
TS28: testis
|
Weak
|
Strong
|
Not Specified; total RNA |
Not Specified |
| Male |
| P6
|
TS28: testis
|
Weak
|
Strong
|
Not Specified; total RNA |
Not Specified |
| Male |
| Mol. Wt. Marker Lane |
Notes:
|
Analysis Tools
