Dlk1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:91849 Lin SP, et al., Asymmetric regulation of imprinting on the maternal and paternal chromosomes at the Dlk1-Gtl2 imprinted cluster on mouse chromosome 12. Nat Genet. 2003 Sep;35(1):97-102
Assay type:	Northern blot
MGI Accession ID:	MGI:3055962
Gene symbol:	Dlk1
Gene name:	delta like non-canonical Notch ligand 1

Probe:	Dlk1 probe2
Visualized with:	Phosphorimaging
Assay notes:	These data were generated using control and mutant embryos from multiple litters.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
+/+	E16.0 (a)	TS24: embryo	Present (b)	Not Specified	involves: 129P2/OlaHsd * C57BL/6		Not Specified
KO/+	E16.0 (a)	TS24: embryo	Present (c)	Not Specified	involves: 129P2/OlaHsd * C57BL/6	Igdmr^tm1Ace/Igdmr⁺	Not Specified	In this sample the deletion was transmitted maternally.
KO/+	E16.0 (a)	TS24: embryo	Present (c)	Not Specified	involves: 129P2/OlaHsd * C57BL/6	Igdmr^tm1Ace/Igdmr⁺	Not Specified	In this sample the deletion was transmitted maternally.
+/+	E16.0 (a)	TS24: embryo	Present (b)	Not Specified	involves: 129P2/OlaHsd * C57BL/6		Not Specified
+/KO	E16.0 (a)	TS24: embryo	Present (d)	Not Specified	involves: 129P2/OlaHsd * C57BL/6	Igdmr^tm1Ace/Igdmr⁺	Not Specified	In this sample the deletion was transmitted paternally.
+/KO	E16.0 (a)	TS24: embryo	Present (d)	Not Specified	involves: 129P2/OlaHsd * C57BL/6	Igdmr^tm1Ace/Igdmr⁺	Not Specified	In this sample the deletion was transmitted paternally.

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Other experiments showed that expression in the wild type is from the paternal allele.
(c) Expression levels are twice that seen in the wild type. Other experiments showed that this is due to transcription from both alleles.
(d) Expression levels are similar to those seen in wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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