Igdmr^tm1Ace
Targeted Allele Detail

Summary

• Symbol: Igdmr^tm1Ace
• Name: intergenic germline-derived differentially methylated region; targeted mutation 1, Anne C Ferguson-Smith
• MGI ID: MGI:3051685
• Synonyms: deltaIG-DMR, DeltaIG-DMR/loxP, Igdmr^tm1Ace-mat
• Gene: Igdmr Location: unknown Genetic Position: Chr12, Syntenic
• Alliance: Igdmr^tm1Ace page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91849
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The region was replaced by a floxed neomycin resistance cassette, which was subsequently excised via transient Cre expression. Southern blot indicated successful targeting. The maternally inherited deletion results in biallelic expression of Dlk1, and Rtl1, with maternal transcript levels of Rtl1 at ~77% that of the unmanipulated paternal allele. The maternally inherited deletion also resulted in the activation of maternal Dio3. (J:91849)

Expression

In Mice Carrying this Mutation:

32 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igdmr Mutation: 2 strains or lines available

References

• Original: J:91849 Lin SP, et al., Asymmetric regulation of imprinting on the maternal and paternal chromosomes at the Dlk1-Gtl2 imprinted cluster on mouse chromosome 12. Nat Genet. 2003 Sep;35(1):97-102
• All: 6 reference(s)