Gene Expression Data
Assay Details
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Assay
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Reference:
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J:89228
Chen MH, et al., Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates. Genes Dev. 2004 Mar 15;18(6):641-59
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3053414
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Probe:
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En1 probe10
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Probe preparation:
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Antisense labelled with P33 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3I:
embryonic day 10.5
(more  )
Specimen
3I:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
3I
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Expression is in v1 interneurons.
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Specimen
3J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * FVB/N * various
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Mutant Allele(s):
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Hhattm1Ptch/Hhattm1Ptch
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
3J
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In this mutant, expression is shifted toward the ventral midline.
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Specimen
3K:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * FVB/N * various
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Mutant Allele(s):
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Shhtm1Ptch/Shhtm1Ptch
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Trace |
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3K
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In this mutant, most v2 interneurons, motor neurons, and v3 interneurons are missing. Expression annotation is inferred from this.
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Specimen
3L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
Regionally restricted |
3L
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This mutant phenocopies that shown in Fig. 3J. Expression annotation is inferred from this.
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Analysis Tools
