Probe:	c164
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Schematic illustrations of expression patterns are shown in Figure 12.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	5A	Expression is in a zone seprarating the alar and basal plates along the length of the spinal cord and rhombencephalon.
TS15: neural tube	Present	Regionally restricted	5A	Expression is in a zone seprarating the alar and basal plates along the length of the spinal cord and rhombencephalon.
TS15: diencephalon	Present	Regionally restricted	5A	Expression is in the mammillary area and dorsal thalamus to pretectum.
TS15: future midbrain	Present		5A

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	6A	Expression is in three domains rostral to the dorsal thalamus: anterior entopenduncular area, eminentia thalami, and in the optic chiasma, the basal zone of the hypothalamus, mammillary areas and zona limitans intrathalamica.
TS19: telencephalon	Present	Regionally restricted	6A	Expression is in the septum.
TS19: midbrain	Strong	Regionally restricted	6A	Expression is in the dorso-lateral wall.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	6C	Expression is in three domains rostral to the dorsal thalamus: anterior entopenduncular area, eminentia thalami, and in the optic chiasma, the basal zone of the hypothalamus, mammillary areas and zona limitans intrathalamica.
TS19: midbrain	Strong	Regionally restricted	6C	Expression is in the dorso-lateral wall and in the isthmus.
TS19: hindbrain	Strong	Regionally restricted	6C	Expression is at the isthmus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	6E	Expression is in three domains rostral to the dorsal thalamus: anterior entopenduncular area, eminentia thalami, and in the optic chiasma, the basal zone of the hypothalamus, mammillary areas and zona limitans intrathalamica.
TS19: midbrain	Strong	Regionally restricted	6E	Expression is in the dorso-lateral wall.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	7A	Expression is in three domains rostral to the dorsal thalamus: anterior entopenduncular area, eminentia thalami, and in the optic chiasma, the basal zone of the hypothalamus, mammillary areas and zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS19: neural tube alar columns	Present	Regionally restricted	7C	Expression is in a zone seprarating the alar and basal plates along the length of the spinal cord.
TS19: neural tube basal columns	Present	Regionally restricted	7C	Expression is in a zone seprarating the alar and basal plates along the length of the spinal cord.
TS19: hindbrain	Present	Regionally restricted	7C	Expression is in a zone seprarating the alar and basal plates along the length of the rhombencephalon.

Structure	Level	Pattern	Image	Note
TS19: neural tube alar columns	Present	Regionally restricted	7E	Expression is in a zone seprarating the alar and basal plates along the length of the spinal cord.
TS19: neural tube basal columns	Present	Regionally restricted	7E	Expression is in a zone seprarating the alar and basal plates along the length of the spinal cord.
TS19: hindbrain	Present	Regionally restricted	7E	Expression is in a zone seprarating the alar and basal plates along the length of the rhombencephalon.
TS19: telencephalon	Present	Regionally restricted	7E	Expression is in the caudal ganglionic eminence.
TS19: diencephalon	Present	Regionally restricted	7E	Expression is in the eminentia thalami.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	9C	Along the length of the neural tube expression is in the alar and basal plates.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present		9D
TS20: alar columns	Present	Regionally restricted	9D	Along the length of the neural tube expression is as a bilateral pair of narrow bands at the boundary between the alar and basal plates.
TS20: basal columns	Present	Regionally restricted	9D	Along the length of the neural tube expression is as a bilateral pair of narrow bands at the boundary between the alar and basal plates.