Antibody:	BEN antibody
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present	Uniform

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present			Staining is nuclear.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present			Staining is nuclear.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present			Staining is nuclear.

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present			Staining is nuclear.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present			Staining is nuclear.
TS5: trophectoderm	Present			Staining is nuclear.

Structure	Level	Pattern	Image	Note
TS6: trophectoderm	Present			Staining is cytoplasmic.
TS6: embryo	Absent

Structure	Level	Pattern	Image	Note
TS8: embryo endoderm	Present
TS8: extraembryonic endoderm	Present

Structure	Level	Pattern	Image	Note
TS9: embryo endoderm	Present
TS9: extraembryonic endoderm	Present

Structure	Level	Pattern	Image	Note
TS10: allantois	Present
TS10: amniotic fold	Present
TS10: neural ectoderm	Present

Structure	Level	Pattern	Image	Note
TS11: allantois	Present
TS11: amnion	Present
TS11: heart	Present
TS11: neural crest	Present
TS11: neural ectoderm	Present

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present
TS13: neural ectoderm	Present
TS13: somite	Present
TS13: foregut diverticulum	Present
TS13: heart	Present
TS13: lateral plate mesoderm	Present

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Moderate
TS13: neural tube	Moderate
TS13: neural tube floor plate	Present
TS13: neural tube roof plate	Present

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Strong
TS15: future midbrain	Strong
TS15: future hindbrain	Moderate
TS15: neural tube	Strong
TS15: neural tube lateral wall	Present	Regionally restricted		Expression is in the ventricular zone and there is moderate expression in motor neurons and inter neurons.
TS15: neural tube roof plate	Present
TS15: neural tube floor plate	Absent

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong
TS17: diencephalon	Strong
TS17: midbrain	Strong
TS17: hindbrain	Strong			There is moderate expression in the metencephalon and myelencephalon.
TS17: neural tube	Strong
TS17: neural tube lateral wall	Present	Regionally restricted		Expression is in the ventricular zone and there is strong expression in motor neurons and moderate expression in inter neurons.
TS17: neural tube roof plate	Moderate
TS17: neural tube floor plate	Absent

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Moderate
TS19: diencephalon	Moderate	Regionally restricted		Expression is in the thalamus and hypothalamus.
TS19: midbrain	Strong
TS19: cerebellum primordium	Moderate
TS19: metencephalon basal plate	Present
TS19: myelencephalon	Present	Regionally restricted		Expression is in the medulla.
TS19: neural tube	Strong
TS19: neural tube ventricular layer	Present
TS19: neural tube basal columns	Strong	Regionally restricted		Strong expression in motor neurons and moderate expression in inter neurons.
TS19: neural tube roof plate	Moderate
TS19: neural tube floor plate	Absent

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present
TS20: cerebral cortex	Present
TS20: hypothalamus	Present
TS20: thalamus	Present
TS20: midbrain	Moderate
TS20: cerebellum primordium	Present
TS20: pons	Present
TS20: medulla oblongata	Present
TS20: spinal cord	Strong
TS20: spinal cord ventricular layer	Present
TS20: basal columns	Strong	Regionally restricted		Strong expression in motor neurons and moderate expression in inter neurons.
TS20: spinal cord roof plate	Moderate
TS20: spinal cord floor plate	Absent

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present
TS21: cerebral cortex	Present
TS21: hypothalamus	Present
TS21: thalamus	Present
TS21: midbrain	Present
TS21: cerebellum	Present
TS21: pons	Present
TS21: medulla oblongata	Present
TS21: spinal cord	Moderate
TS21: spinal cord ventricular layer	Absent
TS21: basal columns	Present	Regionally restricted		Strong expression in motor neurons and there is expression in inter neurons.
TS21: spinal cord roof plate	Present
TS21: spinal cord floor plate	Absent

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present
TS22: cerebral cortex	Present
TS22: hypothalamus	Present
TS22: thalamus	Present
TS22: midbrain	Present
TS22: cerebellum	Present
TS22: pons	Present
TS22: medulla oblongata	Present
TS22: spinal cord	Present
TS22: spinal cord ventricular layer	Absent
TS22: basal columns	Present	Regionally restricted		Expression is in motor neurons and in inter neurons.
TS22: spinal cord roof plate	Present
TS22: spinal cord floor plate	Absent

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Present
TS23: cerebral cortex	Present
TS23: hypothalamus	Present
TS23: thalamus	Present
TS23: midbrain	Present
TS23: cerebellum	Present
TS23: pons	Present
TS23: medulla oblongata	Absent
TS23: spinal cord	Present
TS23: spinal cord ventricular layer	Absent
TS23: basal columns	Present			Expression is in motor neurons and in inter neurons.
TS23: spinal cord floor plate	Absent
TS23: spinal cord roof plate	Absent

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Absent
TS25: telencephalon	Absent
TS25: hypothalamus	Absent
TS25: thalamus	Absent
TS25: midbrain	Absent
TS25: cerebellum	Absent
TS25: pons	Absent
TS25: medulla oblongata	Absent
TS25: spinal cord	Absent
TS25: basal columns	Absent
TS25: spinal cord floor plate	Absent
TS25: spinal cord roof plate	Absent

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Absent
TS26: telencephalon	Absent
TS26: hypothalamus	Absent
TS26: thalamus	Absent
TS26: midbrain	Absent
TS26: cerebellum	Absent
TS26: pons	Absent
TS26: medulla oblongata	Absent

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present
TS27: hypothalamus	Present
TS27: thalamus	Present
TS27: midbrain	Absent
TS27: cerebellum	Present
TS27: pons	Present
TS27: medulla oblongata	Absent

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Absent
TS28: hypothalamus	Absent
TS28: thalamus	Absent
TS28: cerebellum	Present
TS28: pons	Present
TS28: medulla oblongata	Absent

Structure	Level	Pattern	Image	Note
TS17: lens pit	Strong
TS17: optic cup	Strong			Strong expression in the retina, absent in the inner and outer nuclear layers.
TS17: optic cup outer layer	Strong
TS17: eye	Present	Regionally restricted		Absent in the cornea and eyelid, present in retina and lens pit.

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Strong
TS19: neural retinal epithelium	Strong			Absent in the inner and outer nuclear layers.
TS19: pigmented retinal epithelium	Moderate
TS19: cornea epithelium	Absent			Absent in the eyelid.

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Strong
TS20: neural retinal epithelium	Moderate			Absent in the inner and outer nuclear layers.
TS20: pigmented retinal epithelium	Present
TS20: cornea epithelium	Present			Absent in the eyelid.

Structure	Level	Pattern	Image	Note
TS21: lens	Strong
TS21: neural retinal epithelium	Moderate			Absent in the inner and outer nuclear layers.
TS21: pigmented retinal epithelium	Present
TS21: cornea epithelium	Present
TS21: eyelid	Absent

Structure	Level	Pattern	Image	Note
TS22: lens	Strong
TS22: neural retinal epithelium	Moderate			Absent in the inner and outer nuclear layers.
TS22: pigmented retinal epithelium	Absent
TS22: cornea epithelium	Present
TS22: eyelid	Absent

Structure	Level	Pattern	Image	Note
TS23: lens	Strong
TS23: neural retina	Moderate
TS23: cornea epithelium	Absent
TS23: pigmented retinal epithelium	Absent
TS23: retina nerve fiber layer	Absent
TS23: retina nuclear layer	Absent

Structure	Level	Pattern	Image	Note
TS25: lens	Strong
TS25: retina inner nuclear layer	Absent
TS25: retina outer nuclear layer	Strong
TS25: cornea epithelium	Absent
TS25: eyelid	Absent
TS25: pigmented retinal epithelium	Absent

Structure	Level	Pattern	Image	Note
TS26: lens	Strong
TS26: retina inner nuclear layer	Absent
TS26: retina outer nuclear layer	Strong
TS26: cornea epithelium	Absent
TS26: eyelid	Absent
TS26: pigmented retinal epithelium	Absent
TS26: iris	Present

Structure	Level	Pattern	Image	Note
TS27: lens	Strong
TS27: retina	Strong	Regionally restricted		Expression is in the outer nuclear layer, expression is absent in the inner nuclear layer and pigmented retinal epithelium.
TS27: eyelid	Absent

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Moderate	Regionally restricted		Expression is in the oral epithelium/dental epithelium and in the dental mesenchyme/dental papilla. Absent in the inner and outer dental epithelium and the primary and secondary enamel knots.
TS17: 1st branchial arch maxillary component	Moderate	Regionally restricted		Expression is in the oral epithelium/dental epithelium and in the dental mesenchyme/dental papilla. Absent in the inner and outer dental epithelium and the primary and secondary enamel knots.

Structure	Level	Pattern	Image	Note
TS19: mandibular process	Moderate	Regionally restricted		Expression is in the oral epithelium/dental epithelium and in the dental mesenchyme/dental papilla. Absent in the inner and outer dental epithelium and the primary and secondary enamel knots.
TS19: maxillary process	Moderate	Regionally restricted		Expression is in the oral epithelium/dental epithelium and in the dental mesenchyme/dental papilla. Absent in the inner and outer dental epithelium and the primary and secondary enamel knots.

Structure	Level	Pattern	Image	Note
TS20: mandibular process	Moderate	Regionally restricted		Expression is in the oral epithelium/dental epithelium and in the dental mesenchyme/dental papilla. Absent in the inner and outer dental epithelium and the primary and secondary enamel knots.
TS20: maxillary process	Moderate	Regionally restricted		Expression is in the oral epithelium/dental epithelium and in the dental mesenchyme/dental papilla. Absent in the inner and outer dental epithelium and the primary and secondary enamel knots.

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Moderate	Regionally restricted		Expression is in the oral epithelium/dental epithelium and in the dental mesenchyme/dental papilla. Absent in the inner and outer dental epithelium and the primary and secondary enamel knots.
TS21: tooth mesenchyme	Moderate	Regionally restricted		Expression is in the oral epithelium/dental epithelium and in the dental mesenchyme/dental papilla. Absent in the inner and outer dental epithelium and the primary and secondary enamel knots.

Structure	Level	Pattern	Image	Note
TS22: oral epithelium	Present
TS22: enamel organ	Absent			Expression is absent in the secondary enamel knot.
TS22: dental papilla	Present
TS22: tooth epithelium	Absent			Absent in the inner and outer dental epithelium.

Structure	Level	Pattern	Image	Note
TS23: oral epithelium	Present
TS23: enamel organ	Absent			Expression is absent in the secondary enamel knot.
TS23: dental papilla	Present
TS23: tooth epithelium	Absent			Expression is absent in the inner and outer dental epithelium.

Structure	Level	Pattern	Image	Note
TS25: tooth epithelium	Strong
TS25: enamel organ	Strong	Regionally restricted		Strong expression in the secondary enamel knot, absent in the primary enamel knot.
TS25: dental papilla	Present
TS25: oral epithelium	Absent

Structure	Level	Pattern	Image	Note
TS26: tooth epithelium	Strong			Strong expression in the outer dental epithelium.
TS26: enamel organ	Strong			Strong expression in the secondary enamel knot, absent in the primary enamel knot.
TS26: dental papilla	Present
TS26: oral epithelium	Absent

Structure	Level	Pattern	Image	Note
TS27: tooth	Present	Regionally restricted		Expression is in the ameloblasts and odontoblasts.
TS27: dental papilla	Present
TS27: enamel organ	Absent
TS27: oral epithelium	Absent

Structure	Level	Pattern	Image	Note
TS13: trigeminal neural crest	Moderate
TS13: future spinal cord neural crest	Absent			Absent in the dorsal ganglion.
TS13: visceral organ system	Present	Regionally restricted		Absent in the lung and bronchial epithelium.
TS13: gut	Moderate
TS13: heart	Moderate
TS13: genitourinary system	Absent			Absent in the urinary bladder and penis.
TS13: head mesenchyme	Strong
TS13: somite	Weak	Regionally restricted		Expression is in cartilage and ribs.
TS13: surface ectoderm	Weak	Regionally restricted		Expression is in the surface ectoderm and is absent in hair follicles.
TS13: embryo	Weak	Regionally restricted		Expression is in the limb.

Structure	Level	Pattern	Image	Note
TS15: trigeminal preganglion	Strong
TS15: dorsal root ganglion	Absent
TS15: lung	Moderate
TS15: lung epithelium	Moderate	Regionally restricted		Expression is in the bronchial epithelium.
TS15: gut	Strong
TS15: visceral organ system	Present	Regionally restricted		Absent in the liver, strong in the gut, moderate in the lung, and weak in the oesophageal region.
TS15: esophageal region	Weak
TS15: heart	Strong
TS15: cardiovascular system	Weak	Regionally restricted		Expression is in the vascular walls.
TS15: genitourinary system	Strong	Regionally restricted		Strong expression in the urogenital ridge, absent in the bladder and penis.
TS15: head mesenchyme	Strong
TS15: axial skeleton	Moderate	Regionally restricted		Expression is in cartilage and ribs.
TS15: limb	Weak
TS15: surface ectoderm	Weak	Regionally restricted		Expression is in the surface ectoderm, but is absent in hair follicles.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Strong
TS17: gut	Strong
TS17: head mesenchyme	Strong
TS17: heart	Strong
TS17: trigeminal ganglion	Strong
TS17: lung	Moderate
TS17: main bronchus epithelium	Moderate
TS17: esophagus	Weak
TS17: limb	Weak
TS17: liver	Weak
TS17: 1st branchial arch mandibular component	Moderate	Regionally restricted		Expression is in the teeth.
TS17: 1st branchial arch maxillary component	Moderate	Regionally restricted		Expression is in the teeth.
TS17: blood vessel	Weak	Regionally restricted		Expression is in the vascular walls.
TS17: genitourinary system	Strong	Regionally restricted		Expression is in the urogenital ridge, and is absent in the bladder and penis.
TS17: skeleton	Moderate	Regionally restricted		Expression is in cartilage and ribs.
TS17: surface ectoderm	Weak	Regionally restricted		Expression is in the surface ectoderm, but is absent in hair follicles.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Strong
TS19: head mesenchyme	Strong
TS19: heart	Strong
TS19: trigeminal ganglion	Strong
TS19: esophagus	Moderate
TS19: liver	Moderate
TS19: lung	Moderate
TS19: stomach	Moderate
TS19: mandibular process	Moderate	Regionally restricted		Expression is in the teeth.
TS19: maxillary process	Moderate	Regionally restricted		Expression is in the teeth.
TS19: blood vessel	Weak	Regionally restricted		Expression is in the vascular walls.
TS19: reproductive system	Strong	Regionally restricted		Expression is in the urogenital ridge, and is absent in the bladder and penis.
TS19: urinary system	Strong	Regionally restricted		Expression is in the urogenital ridge, and is absent in the bladder and penis.
TS19: limb	Weak
TS19: rib pre-cartilage condensation	Weak
TS19: skeleton	Moderate	Regionally restricted		Expression is in cartilage.
TS19: surface ectoderm	Weak	Regionally restricted		Expression is in the surface ectoderm, but is absent in hair follicles.
TS19: intestine	Absent
TS19: lung epithelium	Absent

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Moderate
TS20: dorsal root ganglion	Strong
TS20: lung	Moderate
TS20: lobar bronchus	Absent			Absent in the bronchial epithelium.
TS20: liver	Strong
TS20: esophagus	Moderate
TS20: stomach	Moderate
TS20: intestine	Absent
TS20: tongue	Weak
TS20: mandibular process	Moderate	Regionally restricted		Expression is in the teeth.
TS20: maxillary process	Moderate	Regionally restricted		Expression is in the teeth.
TS20: heart	Moderate
TS20: blood vessel	Weak	Regionally restricted		Expression is in the vascular walls.
TS20: metanephros	Moderate
TS20: ovary	Moderate
TS20: testis	Moderate
TS20: male reproductive system	Present	Regionally restricted		Absent in the penis, present in testis.
TS20: urinary system	Present	Regionally restricted		Absent in the bladder, moderate in kidney.
TS20: cranium	Moderate
TS20: cartilage	Moderate
TS20: rib pre-cartilage condensation	Weak
TS20: limb	Weak
TS20: skin	Weak	Regionally restricted		Expression is in the surface ectoderm, but is absent in hair follicles.

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Moderate
TS21: dorsal root ganglion	Strong
TS21: lung	Weak
TS21: lobar bronchus	Absent			Absent in the bronchial epithelium.
TS21: liver	Strong
TS21: esophagus	Weak
TS21: stomach	Moderate
TS21: intestine	Absent
TS21: tongue	Weak
TS21: tooth	Moderate
TS21: heart	Weak
TS21: blood vessel	Absent
TS21: metanephros	Weak
TS21: ovary	Weak
TS21: testis	Weak
TS21: male reproductive system	Weak	Regionally restricted		Absent in the penis, weak in testis.
TS21: urinary system	Weak	Regionally restricted		Absent in the bladder, weak in kidney.
TS21: vault of skull	Moderate
TS21: cartilage	Moderate
TS21: rib cartilage condensation	Weak
TS21: limb	Weak
TS21: skin	Weak			Expression includes hair follicles.

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Moderate
TS22: dorsal root ganglion	Strong
TS22: lung	Weak
TS22: lobar bronchus	Absent			Absent in the bronchial epithelium.
TS22: liver	Strong
TS22: esophagus	Weak
TS22: stomach	Moderate
TS22: intestine	Absent
TS22: tongue	Weak
TS22: tooth	Moderate
TS22: blood vessel	Absent
TS22: heart	Absent
TS22: metanephros	Weak
TS22: ovary	Weak
TS22: testis	Weak
TS22: bladder	Absent
TS22: male reproductive system	Weak	Regionally restricted		Absent in the penis, weak in testis.
TS22: vault of skull	Weak
TS22: cartilage	Moderate
TS22: rib cartilage condensation	Weak
TS22: limb	Absent
TS22: skin	Weak			Expression includes hair follicles.

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Moderate
TS23: trigeminal ganglion	Moderate
TS23: lung	Weak
TS23: lobar bronchus	Absent			Absent in the bronchial epithelium.
TS23: liver	Strong
TS23: esophagus	Weak
TS23: stomach	Moderate
TS23: intestine	Absent
TS23: tongue	Weak
TS23: tooth	Moderate
TS23: blood vessel	Absent
TS23: heart	Absent
TS23: metanephros	Weak
TS23: ovary	Weak
TS23: testis	Weak
TS23: bladder	Absent
TS23: glans of male genital tubercle	Absent
TS23: vault of skull	Weak
TS23: cartilage	Moderate
TS23: rib	Weak
TS23: limb	Absent
TS23: skin	Weak			Expression includes hair follicles.

Structure	Level	Pattern	Image	Note
TS25: dorsal root ganglion	Weak
TS25: tooth	Moderate
TS25: blood vessel	Absent
TS25: heart	Absent

Structure	Level	Pattern	Image	Note
TS27: bronchiole epithelium	Weak
TS27: bronchus epithelium	Weak
TS27: liver	Strong
TS27: tooth	Moderate
TS27: heart	Weak
TS27: metanephros	Weak
TS27: epidermis	Weak
TS27: hair follicle	Weak

Structure	Level	Pattern	Image	Note
TS28: bronchiole epithelium	Weak
TS28: bronchus epithelium	Weak
TS28: liver	Strong
TS28: heart	Weak
TS28: metanephros	Weak
TS28: testis	Absent
TS28: epidermis	Weak
TS28: hair follicle	Weak

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted		Expression is in the ventrolateral region.
TS13: oral region	Present	Regionally restricted		Expression is in Rathke's pouch.
TS13: future brain	Present	Regionally restricted		Expression is in the neurons of the cortical and basal layers of the developing brain.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted		Expression is in the ventrolateral region.
TS15: Rathke's pouch	Present
TS15: future brain	Present	Regionally restricted		Expression is in the neurons of the cortical and basal layers of the developing brain.
TS15: branchial arch	Present	Regionally restricted		Expression is in the mesenchyme.
TS15: limb	Present	Regionally restricted		Expression is in the mesenchyme.
TS15: notochord	Present

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted		Expression is in the ventrolateral region.
TS17: Rathke's pouch	Present
TS17: brain	Present	Regionally restricted		Expression is in the neurons of the cortical and basal layers of the developing brain.
TS17: branchial arch	Present	Regionally restricted		Expression is in the mesenchyme.
TS17: limb	Present	Regionally restricted		Expression is in the mesenchyme.
TS17: notochord	Present

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted		Expression is in the ventrolateral region.
TS19: Rathke's pouch	Present
TS19: brain	Present	Regionally restricted		Expression is in the neurons of the cortical and basal layers of the developing brain.
TS19: stomach epithelium	Present
TS19: stomach mesenchyme	Present
TS19: pancreas primordium	Present	Regionally restricted		Expression is in the mesenchyme.
TS19: branchial arch	Present	Regionally restricted		Expression is in the mesenchyme.
TS19: limb	Present	Regionally restricted		Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: brain	Weak			The authors state that expression decreases from E12.5-E15.5.
TS20: tongue	Present	Regionally restricted		Expression is at the foundation of the developing tongue.

Structure	Level	Pattern	Image	Note
TS21: brain	Weak			The authors state that expression decreases from E12.5-E15.5.

Structure	Level	Pattern	Image	Note
TS22: brain	Weak			The authors state that expression decreases from E12.5-E15.5.
TS22: heart	Absent

Structure	Level	Pattern	Image	Note
TS23: brain	Weak			The authors state that expression decreases from E12.5-E15.5.
TS23: spinal cord	Weak

Structure	Level	Pattern	Image	Note
TS24: brain	Weak
TS24: spinal cord	Weak

Structure	Level	Pattern	Image	Note
TS25: brain	Absent
TS25: spinal cord	Absent

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Absent
TS27: tooth	Present	Regionally restricted		Expression is in the dental papilla and in differentiating odontoblasts.
TS27: lung	Present	Regionally restricted		Expression is in the mesenchyme.