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Gene Expression Data
Assay Details
Assay
Reference: J:14393 Robertson M, et al., Expression of alternative forms of differentiation inhibiting activity (DIA/LIF) during murine embryogenesis and in neonatal and adult tissues. Dev Genet. 1993;14(3):165-73
Assay type: RNase protection
MGI Accession ID: MGI:2681114
Gene symbol: Lif
Gene name: leukemia inhibitory factor
Probe: DIA/LIF probe
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Assay notes: Author states that strains C57BL/6, 129, MF1 and PO albino outbreds were used with no differences in expression observed between strains.
Results Image: 4_upper panel
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
Skin 16.5 E16.5 TS24: skin Present (b) 15 µg; total RNA Not Specified Not Specified
Skin 18.5 E18.5 TS26: skin Present (b) 15 µg; total RNA Not Specified Not Specified
Skin NN P newborn TS27: skin Present (b) 15 µg; total RNA Not Specified Not Specified
Skin Ad P adult TS28: skin Present (b) 15 µg; total RNA Not Specified Not Specified
Kidney 13.5 E13.5 TS21: metanephros Present 15 µg; total RNA Not Specified Not Specified
Kidney NN P newborn TS27: metanephros Absent 15 µg; total RNA Not Specified Not Specified
Calvaria 14.5 E14.5 TS22: chondrocranium Present (c) 15 µg; total RNA Not Specified Not Specified
Calvaria 16.5 E16.5 TS24: chondrocranium Present (c) 15 µg; total RNA Not Specified Not Specified
Calvaria NN P newborn TS27: cranium Present (c) 15 µg; total RNA Not Specified Not Specified
Spl NN P newborn TS27: spleen Not Specified 15 µg; total RNA Not Specified Not Specified
Brain 14.5 E14.5 TS22: brain Present (d) 15 µg; total RNA Not Specified Not Specified
Brain 16.5 E16.5 TS24: brain Present (d) 15 µg; total RNA Not Specified Not Specified
Brain 18.5 E18.5 TS26: brain Present (d) 15 µg; total RNA Not Specified Not Specified
Brain NN P newborn TS27: brain Present (d) 15 µg; total RNA Not Specified Not Specified
Brain Ad P adult TS28: brain Present (d) 15 µg; total RNA Not Specified Not Specified
Heart 14.5 E14.5 TS22: heart Not Specified 15 µg; total RNA Not Specified Not Specified
Heart 16.5 E16.5 TS24: heart Not Specified 15 µg; total RNA Not Specified Not Specified
Heart NN P newborn TS27: heart Present (e) 15 µg; total RNA Not Specified Not Specified
Heart Ad P adult TS28: heart Not Specified 15 µg; total RNA Not Specified Not Specified
Notes:
(a) Authors state that there are 2 transcripts, D and M of 368 and 345bp, respectively.
(b) Expression in skin increased through development and continued into the adult.
(c) Expression of the D transcript in calvaria increased from E14.5 to neonatal, but the M transcript level was unchanged.
(d) Expression in brain increased through development and continued into the adult. Both the D and M transcripts were present in the embryo and neonate, but only the M transcript was present in adult.
(e) Expression in heart was maximum in the neonatal sample.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory