About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:14393 Robertson M, et al., Expression of alternative forms of differentiation inhibiting activity (DIA/LIF) during murine embryogenesis and in neonatal and adult tissues. Dev Genet. 1993;14(3):165-73
Assay type: RNase protection
MGI Accession ID: MGI:2681112
Gene symbol: Lif
Gene name: leukemia inhibitory factor
Probe: DIA/LIF probe
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Assay notes: Author states that strains C57BL/6, 129, MF1 and PO albino outbreds were used with no differences in expression observed between strains.
Results Image: 3
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
Placenta 12.5 E12.5 TS28: placenta Present 15 µg; total RNA Not Specified Female
Placenta 14.5 E14.5 TS28: placenta Present 15 µg; total RNA Not Specified Female
Amnion 12.5 E12.5 TS20: extraembryonic component Present 15 µg; total RNA Not Specified Not Specified
Amnion 14.5 E14.5 TS22: extraembryonic component Weak 15 µg; total RNA Not Specified Not Specified
Amnion 16.5 E16.5 TS24: extraembryonic component Weak 15 µg; total RNA Not Specified Not Specified
tRNA (D) Control
tRNA (D+G) Control
Embryo 8.5 E8.5 TS13: embryo Trace 15 µg; total RNA Not Specified Not Specified
Embryo 9.5 E9.5 TS15: embryo Absent 15 µg; total RNA Not Specified Not Specified
Embryo 10.5 E10.5 TS17: embryo Absent 15 µg; total RNA Not Specified Not Specified
Vis Yolk Sac 9.5 E9.5 TS15: yolk sac Weak 15 µg; total RNA Not Specified Not Specified
Vis Yolk Sac 10.5 E10.5 TS17: yolk sac Absent 15 µg; total RNA Not Specified Not Specified
Vis Yolk Sac 14.5 E14.5 TS22: yolk sac Absent 15 µg; total RNA Not Specified Not Specified
Dec 9.5 E9.5 TS28: decidua Present 15 µg; total RNA Not Specified Female
12.5 d Visc E12.5 TS20: yolk sac Weak (b) 15 µg; total RNA Not Specified Not Specified
12.5 d Liv E12.5 TS20: liver Not Specified 15 µg; total RNA Not Specified Not Specified
12.5 d Br E12.5 TS20: brain Not Specified 15 µg; total RNA Not Specified Not Specified
14.5 d Liv E14.5 TS22: liver Not Specified 15 µg; total RNA Not Specified Not Specified
Notes:
(a) Authors state that there are 2 transcripts, D and M of 368 and 345bp, respectively.
(b) Expression was detected of the M transcript only in E12.5 viscera.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory