Fgf8 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:83255 Chi CL, et al., The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum. Development. 2003 Jun;130(12):2633-44
Assay type:	RNA in situ
MGI Accession ID:	MGI:2677300
Gene symbol:	Fgf8
Gene name:	fibroblast growth factor 8

Probe:	Fgf8 ex3 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 1D, control, 7s: embryonic day 8.0 (more

)
Note: 7 somites. Littermate control; exact genotype not specified.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	1D control 7s	Authors report expression in mesencephalon/metencephalon.
TS12: future midbrain	Present	Regionally restricted	1D control 7s	Authors report expression in mesencephalon/metencephalon.

1D MHB KO 7s ©

Specimen 1D, MHB KO, 7s: embryonic day 8.0; Fgf8^tm1.3Mrt/Fgf8^tm1.4Mrt, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant. 7 somites.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		1D MHB KO 7s	From the 3-5 somite stage, expression levels in mutants are comparable those those seen in controls. From the 6-8 somite stage, expression is present at lower levels than in the control embryos.
TS12: future midbrain	Present		1D MHB KO 7s	From the 3-5 somite stage, expression levels in mutants are comparable those those seen in controls. From the 6-8 somite stage, expression is present at lower levels than in the control embryos.

Specimen 1D, control, 11s (left): embryonic day 8.5 (more

)
Note: 11 somites. Littermate control; exact genotype not specified.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	1D control 11s (left)	Authors report expression in mesencephalon/metencephalon.
TS13: future midbrain	Present	Regionally restricted	1D control 11s (left)	Authors report expression in mesencephalon/metencephalon.

Specimen 1D, MHB KO, 11s (left): embryonic day 8.5; Fgf8^tm1.3Mrt/Fgf8^tm1.4Mrt, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant. 11 somites.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Absent		1D MHB KO 11s (left)	From the 10 somite stage onward, expression is not detected in the mutants.
TS13: future midbrain	Absent		1D MHB KO 11s (left)	From the 10 somite stage onward, expression is not detected in the mutants.

Specimen 1D, control, 11s (right): embryonic day 8.5 (more

)
Note: 11 somites. Littermate control; exact genotype not specified.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	1D control 11s (right)	Authors report expression in mesencephalon/metencephalon.
TS13: future midbrain	Present	Regionally restricted	1D control 11s (right)	Authors report expression in mesencephalon/metencephalon.

Specimen 1D, MHB KO, 11s (right): embryonic day 8.5; Fgf8^tm1.3Mrt/Fgf8^tm1.4Mrt, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant. 11 somites.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Absent		1D MHB KO 11s (right)	From the 10 somite stage onward, expression is not detected in the mutants.
TS13: future midbrain	Absent		1D MHB KO 11s (right)	From the 10 somite stage onward, expression is not detected in the mutants.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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