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Gene Expression Data
Assay Details
Assay
Reference: J:83255 Chi CL, et al., The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum. Development. 2003 Jun;130(12):2633-44
Assay type: RNA in situ
MGI Accession ID: MGI:2677299
Gene symbol: En1
Gene name: engrailed 1
Probe: En1 probe7
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1B-2s: embryonic day 8.0 (more )
Note: 2 somites.
Structure Level Pattern Image Note
TS12: future hindbrain Present Regionally restricted 1B 2s Authors report expression in the midbrain-hindbrain boundary region.
TS12: future midbrain Present Regionally restricted 1B 2s Authors report expression in the midbrain-hindbrain boundary region.
Specimen 1B-5s: embryonic day 8.0 (more )
Note: 5 somites.
Structure Level Pattern Image Note
TS12: future hindbrain Present Regionally restricted 1B 5s Authors report expression in the midbrain-hindbrain boundary region.
TS12: future midbrain Present Regionally restricted 1B 5s Authors report expression in the midbrain-hindbrain boundary region.
Specimen 1B-10s: embryonic day 8.5 (more )
Note: 10 somites.
Structure Level Pattern Image Note
TS13: future hindbrain Present Regionally restricted 1B 10s Authors report expression in the midbrain-hindbrain boundary region.
TS13: future midbrain Present Regionally restricted 1B 10s Authors report expression in the midbrain-hindbrain boundary region.
Specimen 5M: embryonic day 8.5 (more )
Note: 8 somites. Littermate control; exact genotype not specified.
Structure Level Pattern Image Note
TS13: future brain Present 5M
Specimen 5N: embryonic day 8.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. 7 somites.
Structure Level Pattern Image Note
TS13: future brain Present 5N Expression levels are at slightly lower levels than those seen in controls.
Specimen 5U: embryonic day 9.0 (more )
Note: 14 somites. Littermate control; exact genotype not specified.
Structure Level Pattern Image Note
TS14: future brain Present 5U
Specimen 5V: embryonic day 9.0; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. 14 somites.
Structure Level Pattern Image Note
TS14: future brain Weak Regionally restricted 5V In the mutant, expression is present in only a small patch.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory