Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:79394
Ishibashi M, et al., A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development. 2002 Oct;129(20):4807-19
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2676611
|
|
Gene symbol:
|
Wnt7b
|
|
Gene name:
|
wingless-type MMTV integration site family, member 7B
|
|
Probe:
|
Wnt7b probe2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
2I:
embryonic day 9.5
(more  )
Note: 21 somite stage.
Specimen
2I:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Embryonic age was assigned based on somite numbers reported in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
21 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: diencephalon |
Present |
Regionally restricted |
2I
|
Expression in the dorsal telencephalon and anterior diencephalon.
|
| TS15: telencephalon |
Present |
Regionally restricted |
2I
|
Expression in the dorsal telencephalon and anterior diencephalon.
|
Specimen
2J:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
|
Mutant Allele(s):
|
Shhtm1Amc/Shhtm1Amc
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Embryonic age was assigned based on somite numbers reported in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
21 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: diencephalon |
Present |
Regionally restricted |
2J
|
Expressed in the same pattern as in wild type; diencephalic domain diminished.
|
| TS15: telencephalon |
Present |
Regionally restricted |
2J
|
Expressed in the same pattern as in wild type; diencephalic domain diminished.
|
|
Analysis Tools